Evolution and Expression Patterns of the Fructose 1,6-Bisphosptase Gene Family in a Miracle Tree (Neolamarckia cadamba)

Qingmin Que; Xiaohan Liang; Huiyun Song; Chunmei Li; Pei Li; Ruiqi Pian; Xiaoyang Chen; Wei Zhou; Kunxi Ouyang

doi:10.3390/genes13122349

Evolution and Expression Patterns of the Fructose 1,6-Bisphosptase Gene Family in a Miracle Tree (Neolamarckia cadamba)

Genes (Basel). 2022 Dec 13;13(12):2349. doi: 10.3390/genes13122349.

Authors

Qingmin Que¹, Xiaohan Liang¹, Huiyun Song¹, Chunmei Li², Pei Li¹, Ruiqi Pian¹, Xiaoyang Chen¹, Wei Zhou¹, Kunxi Ouyang³

Affiliations

¹ College of Forestry and Landscape Architecture, South China Agricultural University, Wushan Road 483, Tianhe District, Guangzhou 510642, China.
² Zhaoqing Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Zhaoqing 526238, China.
³ Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Wushan Road 483, Tianhe District, Guangzhou 510642, China.

Abstract

Neolamarckia cadamba (N. cadamba) is a fast-growing tree species with tremendous economic and ecological value; the study of the key genes regulating photosynthesis and sugar accumulation is very important for the breeding of N. cadamba. Fructose 1,6-bisphosptase (FBP) gene has been found to play a key role in plant photosynthesis, sugar accumulation and other growth processes. However, no systemic analysis of FBPs has been reported in N. cadamba. A total of six FBP genes were identifed and cloned based on the N. cadamba genome, and these FBP genes were sorted into four groups. The characteristics of the NcFBP gene family were analyzed such as phylogenetic relationships, gene structures, conserved motifs, and expression patterns. A cis-acting element related to circadian control was first found in the promoter region of FBP gene. Phylogenetic and quantitative real-time PCR analyses showed that NcFBP5 and NcFBP6 may be chloroplast type 1 FBP and cytoplasmic FBP, respectively. FBP proteins from N. cadamba and 22 other plant species were used for phylogenetic analyses, indicating that FBP family may have expanded during the evolution of algae to mosses and differentiated cpFBPase1 proteins in mosses. This work analyzes the internal relationship between the evolution and expression of the six NcFBPs, providing a scientific basis for the evolutionary pattern of plant FBPs, and promoting the functional studies of FBP genes.

Keywords: Neolamarckia cadamba; bioinformatics analysis; fructose 1,6-bisphosptase; phylogenetic analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fructose*
Phylogeny
Plant Breeding*

Substances

Fructose

Grants and funding

This research was funded by “Forestry Science and Technology Innovation Project in Guangdong Province [2023KJCX005, 2018KJCX001]” and “The National Key R & D Program of China during the 14th Five-Year Plan Period [2022YFD2200205]”.