Apple Derived Exosomes Improve Collagen Type I Production and Decrease MMPs during Aging of the Skin through Downregulation of the NF-κB Pathway as Mode of Action

Martina Trentini; Ilaria Zanolla; Federica Zanotti; Elena Tiengo; Danilo Licastro; Simeone Dal Monego; Luca Lovatti; Barbara Zavan

doi:10.3390/cells11243950

Apple Derived Exosomes Improve Collagen Type I Production and Decrease MMPs during Aging of the Skin through Downregulation of the NF-κB Pathway as Mode of Action

Cells. 2022 Dec 7;11(24):3950. doi: 10.3390/cells11243950.

Authors

Martina Trentini¹, Ilaria Zanolla¹, Federica Zanotti¹, Elena Tiengo¹, Danilo Licastro², Simeone Dal Monego², Luca Lovatti¹, Barbara Zavan¹

Affiliations

¹ Department Translational Medicine, University of Ferrara, 44121 Ferrara, Italy.
² AREA Science Park, Padriciano 99, 34149 Trieste, Italy.

Abstract

Skin ageing is strictly related to chronic inflammation of the derma and the decay of structural proteins of the extracellular matrix. Indeed, it has become common practice to refer to this phenomenon as inflammageing. Biotech innovation is always in search of new active principles that induce a youthful appearance. In this paper, apple-derived nanovesicles (ADNVs) were investigated as novel anti-inflammatory compounds, which are able to alter the extracellular matrix production of dermal fibroblasts. Total RNA sequencing analysis revealed that ADNVs negatively influence the activity of Toll-like Receptor 4 (TLR4), and, thus, downregulate the NF-κB pro-inflammatory pathway. ADNVs also reduce extracellular matrix degradation by increasing collagen synthesis (COL3A1, COL1A2, COL8A1 and COL6A1) and downregulating metalloproteinase production (MMP1, MMP8 and MMP9). Topical applications for skin regeneration were evaluated by the association of ADNVs with hyaluronic-acid-based hydrogel and patches.

Keywords: apple; exosomes; extracellular matrix; inflammageing; wound healing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Collagen Type I / metabolism
Down-Regulation
Exosomes* / metabolism
Malus* / metabolism
Matrix Metalloproteinases / metabolism
NF-kappa B / metabolism

Substances

Collagen Type I, alpha2 Subunit
Collagen Type I
NF-kappa B
Matrix Metalloproteinases

Grants and funding

This project was financed by Sistema Argo, Area Science Park, The Potential Use of Exosomes as Immunomodulatory Agents for COVID-19 Patients.