Label-Free Myoglobin Biosensor Based on Pure and Copper-Doped Titanium Dioxide Nanomaterials

Ahmad Umar; Mazharul Haque; Shafeeque G Ansari; Hyung-Kee Seo; Ahmed A Ibrahim; Mohsen A M Alhamami; Hassan Algadi; Zubaida A Ansari

doi:10.3390/bios12121151

Label-Free Myoglobin Biosensor Based on Pure and Copper-Doped Titanium Dioxide Nanomaterials

Biosensors (Basel). 2022 Dec 8;12(12):1151. doi: 10.3390/bios12121151.

Authors

Ahmad Umar^{1

2

3}, Mazharul Haque⁴, Shafeeque G Ansari⁴, Hyung-Kee Seo⁵, Ahmed A Ibrahim^{1

2}, Mohsen A M Alhamami¹, Hassan Algadi^{2

6}, Zubaida A Ansari⁴

Affiliations

¹ Department of Chemistry, Faculty of Science and Arts, Najran University, Najran 11001, Saudi Arabia.
² Promising Centre for Sensors and Electronic Devices (PCSED), Najran University, Najran 11001, Saudi Arabia.
³ Department of Materials Science and Engineering, The Ohio State University, Columbus, OH 43210, USA.
⁴ Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi 110025, India.
⁵ School of Chemical Engineering, Jeonbuk National University, Jeonju 56212, Republic of Korea.
⁶ Department of Electrical Engineering, College of Engineering, Najran University, Najran 11001, Saudi Arabia.

Abstract

In this study, using pure and copper-doped titanium dioxide (Cu-TiO₂) nanostructures as the base matrix, enzyme-less label free myoglobin detection to identify acute myocardial infarction was performed and presented. The Cu-TiO₂ nanomaterials were prepared using facile sol-gel method. In order to comprehend the morphologies, compositions, structural, optical, and electrochemical characteristics, the pure and Cu-TiO₂ nanomaterials were investigated by several techniques which clearly revealed good crystallinity and high purity. To fabricate the enzyme-less label free biosensor, thick films of synthesized nanomaterials were applied to the surface of a pre-fabricated gold screen-printed electrode (Au-SPE), which serves as a working electrode to construct the myoglobin (Mb) biosensors. The interference study of the fabricated biosensor was also carried out with human serum albumin (HSA) and cytochrome c (cyt-c). Interestingly, the Cu-doped TiO₂ nanomaterial-based Mb biosensor displayed a higher sensitivity of 61.51 µAcm^-2/nM and a lower detection limit of 14 pM with a response time of less than 10 ms.

Keywords: Cu-doped TiO2; acute myocardial infarction; electrochemical biosensor; myoglobin.

MeSH terms

Biosensing Techniques* / methods
Copper
Electrochemical Techniques / methods
Humans
Myoglobin
Nanostructures*
Titanium / chemistry

Substances

titanium dioxide
Myoglobin
Copper
Titanium

Grants and funding

The authors are thankful to the Deanship of Scientific Research at Najran University, Najran, Kingdom of Saudi Arabia for funding this work under National Priority Research project grant number NU/NRP/SERC/11/1.