Electrochemiluminescence Aptasensor Based on Gd(OH)3 Nanocrystalline for Ochratoxin A Detection in Food Samples

Chunyuan Tian; Minggang Wei; Xiaobin Wang; Qing Hua; Feiyan Tang; Lijun Zhao; Xuming Zhuang; Feng Luan

doi:10.3390/bios12121141

Electrochemiluminescence Aptasensor Based on Gd(OH)₃ Nanocrystalline for Ochratoxin A Detection in Food Samples

Biosensors (Basel). 2022 Dec 7;12(12):1141. doi: 10.3390/bios12121141.

Authors

Chunyuan Tian¹, Minggang Wei¹, Xiaobin Wang¹, Qing Hua¹, Feiyan Tang¹, Lijun Zhao¹, Xuming Zhuang¹, Feng Luan¹

Affiliation

¹ College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China.

Abstract

In the present study, the electrochemiluminescence (ECL) properties of Gd(OH)₃ nanocrystals with K₂S₂O₈ as the cathode coreactant were studied for the first time. Based on the prominent ECL behavior of this material and the excellent specificity of the aptamer technique, an ECL aptasensor for the detection of ochratoxin A (OTA) was formulated successfully. Over an OTA concentration range of 0.01 pg mL^-1 to 10 ng mL^-1, the change in the ECL signal was highly linear with the OTA concentration, and the limit of detection (LOD) was 0.0027 pg mL^-1. Finally, the ECL aptasensor was further used to detect OTA in real samples (grapes and corn) and satisfactory results were obtained, which indicated that the built method is expected to be applied in food detection.

Keywords: Gd(OH)3 nanocrystals; aptasensor; electrochemiluminescence; food detection; ochratoxin A.

MeSH terms

Aptamers, Nucleotide* / chemistry
Biosensing Techniques* / methods
Electrochemical Techniques / methods
Limit of Detection
Nanoparticles*
Ochratoxins* / analysis

Substances

ochratoxin A
Aptamers, Nucleotide
Ochratoxins

Grants and funding

Grant number 21778047/National Natural Science Foundation of China