The sulfated polysaccharides from cystocarpic plants of Mazzaella parksii were studied. Fractionation at a given KCl concentration allowed us to assume, and stepwise fractionation to prove, that these polysaccharides consisted of several carrageenans that differed in structure and molecular weight. As a result of stepwise fractionation with KCl, nine gelling (1-9) and one non-gelling (10) fractions were obtained. Using IR spectroscopy, it was shown that fractions 3, 4 and 5 were kappa/iota-, kappa- and kappa/beta-carrageenans, respectively. The structures of the main fractions 1, 2, 9 and 10 were investigated in more detail by methylation, NMR spectroscopy and mass spectrometry. Fractions 1 and 2 were hybrid kappa/iota-carrageenans with kappa:iota ratio 79:21 and 63:37, respectively. At the same time, fraction 9 contained kappa-, iota- and small amounts of nu-carrageenans. The fraction 10 had complex structure and was built from kappa-, iota-, beta-, mu- and nu-carrageenans and included agar-like structure, which explained the inability of this fraction to gel at 15 % KCl. It was shown that isolated polysaccharides activated the classical pathway of complement system, increasing the concentration of C1 inhibitor of serine protease by 50 % compared with the negative control.
Keywords: Complement system; Mazzaella parksii; Polysaccharides; Structure.
Copyright © 2022 Elsevier B.V. All rights reserved.