Prediction of DNA rejoining kinetics and cell survival after proton irradiation for V79 cells using Geant4-DNA

Dousatsu Sakata; Ryoichi Hirayama; Wook-Geun Shin; Mauro Belli; Maria A Tabocchini; Robert D Stewart; Oleg Belov; Mario A Bernal; Marie-Claude Bordage; Jeremy M C Brown; Milos Dordevic; Dimitris Emfietzoglou; Ziad Francis; Susanna Guatelli; Taku Inaniwa; Vladimir Ivanchenko; Mathieu Karamitros; Ioanna Kyriakou; Nathanael Lampe; Zhuxin Li; Sylvain Meylan; Claire Michelet; Petteri Nieminen; Yann Perrot; Ivan Petrovic; Jose Ramos-Mendez; Aleksandra Ristic-Fira; Giovanni Santin; Jan Schuemann; Hoang N Tran; Carmen Villagrasa; Sebastien Incerti

doi:10.1016/j.ejmp.2022.11.012

Prediction of DNA rejoining kinetics and cell survival after proton irradiation for V79 cells using Geant4-DNA

Phys Med. 2023 Jan:105:102508. doi: 10.1016/j.ejmp.2022.11.012. Epub 2022 Dec 20.

Authors

Dousatsu Sakata¹, Ryoichi Hirayama², Wook-Geun Shin³, Mauro Belli⁴, Maria A Tabocchini⁵, Robert D Stewart⁶, Oleg Belov⁷, Mario A Bernal⁸, Marie-Claude Bordage⁹, Jeremy M C Brown¹⁰, Milos Dordevic¹¹, Dimitris Emfietzoglou¹², Ziad Francis¹³, Susanna Guatelli¹⁴, Taku Inaniwa¹⁵, Vladimir Ivanchenko¹⁶, Mathieu Karamitros¹⁷, Ioanna Kyriakou¹², Nathanael Lampe¹⁸, Zhuxin Li¹⁹, Sylvain Meylan²⁰, Claire Michelet¹⁹, Petteri Nieminen²¹, Yann Perrot²², Ivan Petrovic¹¹, Jose Ramos-Mendez²³, Aleksandra Ristic-Fira¹¹, Giovanni Santin²¹, Jan Schuemann²⁴, Hoang N Tran¹⁹, Carmen Villagrasa²², Sebastien Incerti¹⁹

Affiliations

¹ Department of Accelerator and Medical Physics, Institute for Quantum Medical Science, National Institutes for Quantum Science and Technology (QST), Chiba 263-8555, Japan; Division of Health Sciences, Osaka University, Osaka 565-0871, Japan. Electronic address: sakata.dousatsu@qst.go.jp.
² Department of Charged Particle Therapy Research, Institute for Quantum Medical Science, National Institutes for Quantum Science and Technology (QST), Chiba 263-8555, Japan.
³ Department of Radiation Oncology, Seoul National University Hospital, Seoul 03080, Republic of Korea.
⁴ Independent Researcher, Rome, Italy.
⁵ Istituto Nazionale di Fisica Nucleare (INFN)-Roma 1, Rome, Italy.
⁶ Department of Radiation Oncology, University of Washington, WA 98195-6043, USA.
⁷ Veksler and Baldin Laboratory of High Energy Physics, Joint Institute for Nuclear Research, 141980 Dubna, Russia; Institute of System Analysis and Management, Dubna State University, 141980 Dubna, Russia.
⁸ Instituto de Fisica Gleb Wataghin, Universidade Estadual de Campinas, Campinas, SP, Brazil.
⁹ INSERM, Université Paul Sabatier, UMR 1037, CRCT, Toulouse, France; Université Toulouse III-Paul Sabatier, UMR 1037, CRCT, Toulouse, France.
¹⁰ Department of Physics and Astronomy, Swinburne University of Technology, Hawthorn, Australia; Centre For Medical Radiation Physics, University of Wollongong, Wollongong, Australia; Department of Radiation Science and Technology, Delft University of Technology, The Netherlands.
¹¹ Vinca Institute of Nuclear Sciences - National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia.
¹² Medical Physics Laboratory, Department of Medicine, University of Ioannina, GR 45110, Ioannina, Greece.
¹³ Saint Joseph University of Beirut, UR Mathématiques et Modélisation, Beirut, Lebanon.
¹⁴ Centre For Medical Radiation Physics, University of Wollongong, Wollongong, Australia.
¹⁵ Department of Accelerator and Medical Physics, Institute for Quantum Medical Science, National Institutes for Quantum Science and Technology (QST), Chiba 263-8555, Japan.
¹⁶ Geant4 Associates International Ltd, Hebden Bridge, UK; Tomsk State University, Tomsk, Russia.
¹⁷ Independent Researcher, Bordeaux, France.
¹⁸ Independent Researcher, Victoria, Australia.
¹⁹ Univ. Bordeaux, CNRS, LP2I Bordeaux, UMR 5797, F-33170 Gradignan, France.
²⁰ Independent Researcher, Paris, France.
²¹ ESA-ESTEC, Noordwijk, The Netherlands.
²² IRSN, Institut de Radioprotection et de Surete Nucleaire, 92262 Fontenay-aux-Roses, France.
²³ Department of Radiation Oncology, University of California San Francisco, San Francisco 94143, CA, USA.
²⁴ Physics Division, Department of Radiation Oncology, Massachusetts General Hospital & Harvard Medical School, Boston, MA, USA.

PMID: 36549067
DOI: 10.1016/j.ejmp.2022.11.012

Abstract

Purpose: Track structure Monte Carlo (MC) codes have achieved successful outcomes in the quantitative investigation of radiation-induced initial DNA damage. The aim of the present study is to extend a Geant4-DNA radiobiological application by incorporating a feature allowing for the prediction of DNA rejoining kinetics and corresponding cell surviving fraction along time after irradiation, for a Chinese hamster V79 cell line, which is one of the most popular and widely investigated cell lines in radiobiology.

Methods: We implemented the Two-Lesion Kinetics (TLK) model, originally proposed by Stewart, which allows for simulations to calculate residual DNA damage and surviving fraction along time via the number of initial DNA damage and its complexity as inputs.

Results: By optimizing the model parameters of the TLK model in accordance to the experimental data on V79, we were able to predict both DNA rejoining kinetics at low linear energy transfers (LET) and cell surviving fraction.

Conclusion: This is the first study to demonstrate the implementation of both the cell surviving fraction and the DNA rejoining kinetics with the estimated initial DNA damage, in a realistic cell geometrical model simulated by full track structure MC simulations at DNA level and for various LET. These simulation and model make the link between mechanistic physical/chemical damage processes and these two specific biological endpoints.

Keywords: Cell survival; DNA damage; Geant4-DNA; Monte Carlo simulation.

MeSH terms

Animals
Cell Survival
Cricetinae
DNA / chemistry
DNA Damage*
Kinetics
Monte Carlo Method
Protons*

Substances

Protons
DNA