Using CRL3BPM E3 ligase substrate recognition sites as tools to impact plant development and stress tolerance in Arabidopsis thaliana

Raed Al-Saharin; Sutton Mooney; Nico Dissmeyer; Hanjo Hellmann

doi:10.1002/pld3.474

Using CRL3^BPM E3 ligase substrate recognition sites as tools to impact plant development and stress tolerance in Arabidopsis thaliana

Plant Direct. 2022 Dec 19;6(12):e474. doi: 10.1002/pld3.474. eCollection 2022 Dec.

Authors

Raed Al-Saharin^{1

2}, Sutton Mooney¹, Nico Dissmeyer³, Hanjo Hellmann¹

Affiliations

¹ Washington State University Pullman Washington USA.
² Tafila Technical University Tafila Jordan.
³ Department of Plant Physiology and Protein Metabolism Lab University of Osnabruck Osnabruck Germany.

Abstract

Cullin-based RING E3 ligases that use BTB/POZ-MATH (BPM) proteins as substrate receptors have been established over the last decade as critical regulators in plant development and abiotic stress tolerance. As such they affect general aspects of shoot and root development, flowering time, embryo development, and different abiotic stress responses, such as heat, drought and salt stress. To generate tools that can help to understand the role of CRL3^BPM E3 ligases in plants, we developed a novel system using two conserved protein-binding motifs from BPM substrates to transiently block CRL3^BPM activity. The work investigates in vitro and in planta this novel approach, and shows that it can affect stress tolerance in plants as well as developmental aspects. It thereby can serve as a new tool for studying this E3 ligase in plants.

Keywords: Arabidopsis; BPM; CRL3; E3 ligase; N‐degron; development; stress; ubiquitin.