Multifunctional AuNPs@HRP@FeMOF immune scaffold with a fully automated saliva analyzer for oral cancer screening

Xinyi Wang; Xianyou Sun; Chiyu Ma; Yanchi Zhang; Liubing Kong; Zhuoru Huang; Yanjie Hu; Hao Wan; Ping Wang

doi:10.1016/j.bios.2022.114910

Multifunctional AuNPs@HRP@FeMOF immune scaffold with a fully automated saliva analyzer for oral cancer screening

Biosens Bioelectron. 2023 Feb 15:222:114910. doi: 10.1016/j.bios.2022.114910. Epub 2022 Nov 11.

Authors

Xinyi Wang¹, Xianyou Sun², Chiyu Ma², Yanchi Zhang², Liubing Kong², Zhuoru Huang², Yanjie Hu³, Hao Wan⁴, Ping Wang⁵

Affiliations

¹ Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China; Binjiang Institute of Zhejiang University, Hangzhou, 310053, China.
² Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China.
³ Department of Respiratory Medicine, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 310016, China.
⁴ Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China; Binjiang Institute of Zhejiang University, Hangzhou, 310053, China. Electronic address: wh1816@zju.edu.cn.
⁵ Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China; Binjiang Institute of Zhejiang University, Hangzhou, 310053, China; The MOE Frontier Science Center for Brain Science & Brain-machine Integration, Zhejiang University, Hangzhou, 310027, China. Electronic address: cnpwang@zju.edu.cn.

PMID: 36542992
DOI: 10.1016/j.bios.2022.114910

Abstract

Delayed diagnosis of cancer-causing death is a worldwide concern. General diagnosis methods are invasive, time-consuming, and operation complicated, which are not suitable for preliminary screening. To address these challenges, the sensing platform based on immune scaffold and fully automated saliva analyzer (FASA) was proposed for oral cancer screening for the first time by non-invasive detection of Cyfra21-1 in saliva. Through one-step synthesis method with unique covalent and electrostatic adsorption strategy, AuNPs@HRP@FeMOF immune scaffold features multiple functions including antibody carrier, catalytic activity, and signal amplification. Highly integrated FASA with the immune scaffold provides automatic testing to avoid false-positive results and reduce pretreatment time without any user intervention. Compared with the commercial analyzer, FASA has comparable performance for Cyfra21-1 detection with a detection range of 3.1-50.0 ng/mL and R² of 0.971, and superior features in full automation, high integration, time saving and low cost. Oral cancer patients could be distinguished accurately by the platform with an excellent correlation (R² of 0.904) and average RSD (5.578%) without sample dilution. The proposed platform provides an effective and promising tool for cancer screening in point-of-care applications, which can be further extended for biomarker detection in universal body fluids, disease screening, prognosis review and homecare monitoring.

Keywords: Biomarker analyzer; Cyfra21-1 detection; Immune scaffold; Metal organic framework; Oral cancer.

MeSH terms

Biosensing Techniques* / methods
Early Detection of Cancer
Gold
Humans
Iron / chemistry
Metal Nanoparticles*
Mouth Neoplasms* / diagnosis
Saliva

Substances

antigen CYFRA21.1
Gold
Iron