Delineation of T cell subsets in chronic rhinosinusitis with nasal polyps

Asmaa M Zahran; Omnia El-Badaway; Ibrahim K I Elsayh; Mohamed M Osman

doi:10.14639/0392-100X-N2023

Delineation of T cell subsets in chronic rhinosinusitis with nasal polyps

Acta Otorhinolaryngol Ital. 2022 Oct;42(5):441-449. doi: 10.14639/0392-100X-N2023.

Authors

Asmaa M Zahran¹, Omnia El-Badaway², Ibrahim K I Elsayh³, Mohamed M Osman⁴

Affiliations

¹ Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.
² Department of Medical Microbiology & Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt.
³ Third year Medical student, Faculty of Medicine, Assiut University, Assiut, Egypt.
⁴ Department of Otorhinolaryngology & Head and Neck Surgery, Faculty of Medicine, Assiut University, Assiut, Egypt.

Abstract
in English, Italian

Objectives: Detailed characterisation of CD45RA⁺ and CD45RO⁺ T cell subsets in both CD4⁺ and CD8⁺ cells in blood and tissue of patients with chronic rhinosinusitis with nasal polyps (CRSwNP) is lacking. We aimed to investigate T cell differentiation levels in blood and tissue and correlate this with CT score and white blood cell count.

Methods: This case-control study was conducted on 21 patients with CRSwNP and 20 healthy controls. Lund-Mackay score was used for radiologic staging of chronic rhinosinusitis. T cell subsets were characterised in blood and nasal polyp tissue using CD4, CD8, CCR7, CD45RA, CD45RO, CD27, and CD95 monoclonal antibodies.

Results: Most variations in T cell subsets were detected in tissue rather than blood. A higher level of CD8⁺ T_CM, CD4⁺ T_SCMs and lower level of CD8⁺ T_EM were detected in the blood of CRSwNP patients vs controls. In CRSwNP patients, substantial decrease of tissue CD8⁺ T_Ns, CD8⁺ T_CMs, CD4⁺ T_Ns, and CD4⁺T_SCMs with marked increase in the percentages of T_EMs and T_EMRAs were detected among CD4⁺ and CD8⁺ T cells compared with levels in blood.

Conclusions: The reduction of T_Ns, T_SCMs, and T_CMs and accumulation of T_EMs and T_EMRAs subsets that function as effector cells in sinonasal mucosa, especially CD8⁺ T cells, might indicate the role of immunomodulation of memory/effector T-cells in the pathogenesis of CRSwNP.

Definizione di sottopopolazioni di cellule T nella rinosinusite cronica con poliposi nasale.

Obiettivi: La caratterizzazione delle sottopopolazioni di cellule T CD45RA⁺ e CD45RO⁺ provenienti da sangue e tessuti di pazienti affetti da rinosinusite cronica con poliposi nasale (CRSwNP) è ad oggi incompleta. Scopo del lavoro è definire i livelli di differenziazione di cellule T in campioni ematici e tissutali e correlarli con CT score e conta linfocitaria.

Metodi: Lo studio caso-controllo è stato condotto su 21 pazienti affetti da CRSwNP e 20 controlli. Le sottopopolazioni di cellule T sono state caratterizzate da campioni ematici e tissutali di polipi nasali, utilizzando anticorpi monoclonali per CD4, CD8, CCR7, CD45RA, CD45RO, CD27, e CD95.

Risultati: La maggior parte delle variazioni delle sottopopolazioni di cellule T è stata riscontrata nei campioni tissutali. Un livello superiore di CD8⁺ T_CM, CD4⁺ T_SCMs e un livello inferiore di CD8⁺ T_EM sono stati riscontrati nel sangue di pazienti affetti da CRSwNP. Nei pazienti con CRSwNP sono stati rilevati una riduzione di CD8⁺ T_Ns, CD8⁺ T_CMs, CD4⁺ T_Ns, e CD4⁺T_SCMs tissutali e un marcato incremento percentuale di T_EMs e T_EMRAs tra linfociti T CD4⁺ e CD8⁺ rispetto ai loro livelli ematici.

Conclusioni: La riduzione di sottopopolazioni T_Ns, T_SCMs, and T_CMs e l’accumulo di T_EMs e T_EMRAs con funzione di cellule effettrici all’interno della mucosa nasale potrebbe suggerire il ruolo immunomodulante delle cellule T di memoria ed effettrici nella patogenesi della CRSwNP.

Keywords: T cell; chronic rhinosinusitis; nasal polyps.

MeSH terms

CD8-Positive T-Lymphocytes / pathology
Case-Control Studies
Chronic Disease
Humans
Nasal Polyps* / complications
Nasal Polyps* / pathology
Rhinitis* / complications
Rhinitis* / pathology
T-Lymphocyte Subsets / pathology

Abstract in English, Italian

MeSH terms

Abstract
in English, Italian