Simultaneous nitrification and denitrification in a novel rotating self-aerated biofilm reactor for decentralized wastewater treatment

Abstract

Decentralized wastewater pollution in rural areas has become a serious problem for the rural environment. In this study, a novel rotating self-aerated biofilm reactor was developed for decentralized wastewater treatment without any aeration equipment. After the long-term operation of 110 days, the removal efficiency reached to 96.06 % (COD), 98.06 % (NH₄⁺-N), and 62.58 % (TN) in the last phase. Under high dissolved oxygen level, the simultaneous nitrification-denitrification (SND) maintained at a stable ratio of 62.53 % and the denitrification rates reached over 28.37 mg/L/h. With the organic loading rate increased, key nitrogen functional bacterial communities such as anoxic denitrifiers (Thiothrix, Flavobacterium, Pseudoxanthomonas, Aquimonas and Azoarcus) and aerobic denitrifiers (Hydrogenophaga, Zoogloea and Terrimonas) increased obviously. Overall, microbial analysis and nitrogen metabolism pathway indicated that an integration of SND process was achieved in this single reactor by the combined action of nitrification, denitrification and comammox without any aeration equipment.

Keywords: Anoxic and aerobic denitrifiers; Metagenome; Nitrogen metabolic pathways; Rotating self-aerated biofilm reactor; Simultaneous nitrification–denitrification.