A water-soluble naphthalimide fluorescent probe for Cr2O72- and Fe3+ based on inner filter effect

Abstract

A probe 3 (2-ethoxy-N-(2-(2-(2-hydroxyethoxy)ethyl)-1,3-dioxo-2,3-dihydro-1H-benzo[de] isoquinolin-6-yl)benzamide) that could selectively respond to Cr₂O₇^2- and Fe³⁺ was reported in this paper. The selectivity, pH titration, concentration titration, detection limit, time dependence, quenching constant and recognition mechanism of probe 3 for Cr₂O₇^2- and Fe³⁺ were studied in CH₃CN/HEPES buffer solution. The results showed that Cr₂O₇^2- and Fe³⁺ could rapidly quench the fluorescence of probe 3 through the inner filter effect (IFE). The quenching kept constant after 30 s, and the quenching constants were 7.99 × 10³ L^.mol^-1 and 4.13 × 10³ L^.mol^-1, respectively. The detection limits of probe 3 for Cr₂O₇^2- and Fe³⁺ were 1.15 μmol^.L^-1 and 1.95 μmol^.L^-1, respectively, which were lower than the maximum allowable concentrations in drinking water stipulated by EPA. The determination results of Cr₂O₇^2- and Fe³⁺ in water samples indicated that probe 3 could be used as a potential detection tool in practical applications.

Keywords: Cr(2)O(7)(2-); Fe(3+); Fluorescent probe; Inner filter effect; Naphthalimide.