Hrip1 enhances tomato resistance to yellow leaf curl virus by manipulating the phenylpropanoid biosynthesis and plant hormone pathway

Yijie Dong; Honghui Zhu; Dewen Qiu

doi:10.1007/s13205-022-03426-6

Hrip1 enhances tomato resistance to yellow leaf curl virus by manipulating the phenylpropanoid biosynthesis and plant hormone pathway

3 Biotech. 2023 Jan;13(1):11. doi: 10.1007/s13205-022-03426-6. Epub 2022 Dec 16.

Authors

Yijie Dong^{1

2}, Honghui Zhu¹, Dewen Qiu²

Affiliations

¹ Key Laboratory of Agricultural Microbiomics and Precision Application, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Key Laboratory of Agricultural Microbiome (MARA), State Key Laboratory of Applied Microbiology Southern China, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070 People's Republic of China.
² State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory of Control of Biological Hazard Factors (Plant Origin) for Agri-Product Quality and Safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.

Abstract

Tomato yellow leaf curl virus (TYLCV) causes tremendous losses of tomato worldwide. An elicitor Hrip1, which produced by Alternaria tenuissima, can serve as a pathogen-associated molecular patterns (PAMPs) to trigger the immune defense response in Nicotiana benthamiana. Here, we show that Hrip1 can be targeted to the extracellular space and significantly delayed the development of symptoms caused by TYLCV in tomato. In basis of RNA-seq profiling, we find that 1621 differential expression genes (DEGs) with the opposite expression patterns are enriched in plant response to biotic stress between Hrip1 treatment and TYLCV infection of tomato. Thirty-two known differential expression miRNAs with the opposite expression patterns are identified by small RNA sequencing and the target genes of these miRNAs are significantly enriched in phenylpropanoid biosynthesis, plant hormone signal transduction and peroxisome. Based on the Pearson correlation analysis, 13 negative and 21 positive correlations are observed between differential expression miRNAs and DEGs. These miRNAs, which act as a key mediator of tomato resistance to TYLCV induced by Hrip1, regulate the expression of phenylpropanoid biosynthesis and plant hormone signal transduction-related genes. Taken together, our results provide an insight into tomato resistance to TYLCV induced by PAMP at transcriptional and posttranscriptional levels.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-022-03426-6.

Keywords: Hrip1; Pathogen-associated molecular patterns (PAMPs); Tomato resistance to virus; Tomato yellow leaf curl virus (TYLCV); Transcriptional and posttranscriptional regulation.

© King Abdulaziz City for Science and Technology 2022, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Abstract

Grants and funding