A genetic roadmap to the response to genotoxic agents in human cells

Alberto Ciccia; Roger A Greenberg; Susan P Lees-Miller; Andre Nussenzweig

doi:10.12703/r-01-0000019

A genetic roadmap to the response to genotoxic agents in human cells

Fac Rev. 2022 Nov 21:11:35. doi: 10.12703/r-01-0000019. eCollection 2022.

Authors

Alberto Ciccia¹, Roger A Greenberg², Susan P Lees-Miller³, Andre Nussenzweig⁴

Affiliations

¹ Columbia University Irving Medical Center.
² University of Pennsylvania.
³ University of Calgary.
⁴ National Institutes of Health.

Abstract

To maintain genome fidelity and prevent diseases such as cancer, our cells must constantly detect, and efficiently and precisely repair, DNA damage. Paradoxically, DNA-damaging agents in the form of radiation and chemotherapy are also used to treat cancer. Olivieri et al. used a CRISPR-based screen to identify genes that, when disrupted, lead to sensitivity or resistance to 27 different DNA-damaging agents used in the lab and/or in the clinic to treat cancer patients¹. Their results reveal multiple new genes and connections that regulate these critical DNA damage repair pathways, with implications for basic and clinical research as well as cancer therapy.

Keywords: CRISPR; DNA damage; DNA repair; DNA-damaging agents.

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