Physico-chemical properties of the association of cetyltrimethylammonium bromide and bovine serum albumin mixture in aqueous-organic mixed solvents

Abstract

Herein, we have investigated the association behavior of bovine serum albumin (BSA) and cetyltrimethylammonium bromide (CTAB) using the conductivity method in H₂O and H₂O + organic mixed solvents at different temperatures. The association phenomenon was detected from the deviation of the conductivity changes with enhancing the surfactant concentration and changes of numerous physico-chemical properties, such as CMC, α, β and thermodynamic variables (∆G⁰_m, ∆H⁰_m and ∆S⁰_m). The values of CMC for the CTAB + BSA system in 10 % (v/v) solvents follow the trend: CMC_water < CMC_water+DMSO < CMC_water+AN < CMC_water+DX < CMC_water+DMF. The interaction of BSA with CTAB is notably influenced due to a change of temperature and extent of hydration of BSA and surfactant. The obtained values of -∆G⁰_m manifest that the association of BSA and CTAB mixture is a spontaneous process, while the values of -∆G⁰_m in presence of 10 % (v/v) aq. organic solvents come out in the given sequence: -∆G_m^o (H₂O + DMSO) > ∆G_m^o (H₂O + DMF) > -∆G_m^o (H₂O + DX) > -∆G_m^o (H₂O + AN). The H-bonding, ion-dipole, along with the hydrophobic interactions, are believed to be the binding interactions between BSA and CTAB in the study media.

Keywords: Bovine serum albumin; Organic solvents; Surfactant.