Classification of Pericarpium Citri Reticulatae (Chenpi) age using surface-enhanced Raman spectroscopy

Guoyu Dai; Longxiang Wu; Jianhua Zhao; Qiunong Guan; Haishan Zeng; Ming Zong; Manqin Fu; Caigan Du

doi:10.1016/j.foodchem.2022.135210

Classification of Pericarpium Citri Reticulatae (Chenpi) age using surface-enhanced Raman spectroscopy

Food Chem. 2023 May 15:408:135210. doi: 10.1016/j.foodchem.2022.135210. Epub 2022 Dec 13.

Authors

Guoyu Dai¹, Longxiang Wu², Jianhua Zhao³, Qiunong Guan⁴, Haishan Zeng³, Ming Zong⁵, Manqin Fu⁶, Caigan Du⁷

Affiliations

¹ Department of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada; Department of Urology, Xiangya Hospital, Central South University, Changsha, Hunan, China.
² Department of Urology, Xiangya Hospital, Central South University, Changsha, Hunan, China.
³ Imaging Unit, Integrative Oncology Department, BC Cancer Research Center, Vancouver, BC, Canada.
⁴ Department of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada.
⁵ Department of Clinical Laboratory, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China. Electronic address: zongming@tongji.edu.cn.
⁶ Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, Guangdong, China. Electronic address: fumanqin84@126.com.
⁷ Department of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada. Electronic address: Caigan@mail.ubc.ca.

PMID: 36527916
DOI: 10.1016/j.foodchem.2022.135210

Abstract

Pericarpium Citri Reticulatae (PCR) is used in food and medical herbal formula, and its quality is determined by its age. Raman spectroscopy is a laser technology for molecular fingerprinting. The feasibility of using surface-enhanced Raman spectroscopy (SERS) to determine the PCR age was investigated. The Raman peaks were acquired using a Raman spectrometer with a 785 nm diode laser and were analyzed using principal component analysis (PCA) followed by linear discriminant analysis (PCA-LDA). There were six major peaks at 600, 730, 990, 1370, 1607, and 1742 cm^-1 in the SERS spectra, and their intensity, especially the peak at 1607 cm^-1, was inversely correlated with the PCR age. The different ages of PCR could be correctly classified with over 90 % accuracy by using PCA-LDA based on the SERS spectra. In conclusion, a Raman spectrometer may be used as a novel method to identify the age of PCR products.

Keywords: Chemical fingerprinting; Chenpi; Pericarpium Citri Reticulatae; Raman spectroscopy; Storage age.

MeSH terms

Citrus* / chemistry
Discriminant Analysis
Drugs, Chinese Herbal* / analysis
Spectrum Analysis, Raman

Substances

chenpi
Drugs, Chinese Herbal