Distinctive community assembly enhances the adaptation to extreme environments during hyperthermophilic composting

Abstract

Hyperthermophilic composting (hTC) is a promising technique for solid waste treatment due to its distinctive microbiomes. However, the assembly process of the hTC microbial community remains unclear. We investigated the assembly process of hTC and explored the underlying drivers influencing community assembly in this work by employing conventional thermophilic composting (cTC) as a comparison group. Our results showed that the two composting treatments have different community assembly processes. Especially for the initial and thermophilic phases, hTC is affected by homogeneous dispersal (48%) and homogeneous selection (44%), respectively, while cTC is controlled by undominant (38%) and homogeneous selection (92%), respectively. Furthermore, random forest models and network results suggested that different factors govern the community assembly in these two composting methods. Specifically, the hTC community increases the stability of the thermophilic community via enhancing the interactions of low-abundance taxa with other operational taxonomic units (OTUs) in community assembly. Our results suggested that the distinctive nature of hTC community assembly may be responsible for its adaptation to extreme environments.

Keywords: Co-occurrence network; Composting microbial community; Dehydrated sludge; Rare taxa; Thermophilic adaptation.