Sample preparation for lipid analysis of intra-articular adipose tissue by using matrix-assisted laser desorption/ionization imaging

Mirella J J Haartmans; Britt S R Claes; Kaj S Emanuel; Gabrielle J M Tuijthof; Ron M A Heeren; Pieter J Emans; Berta Cillero-Pastor

doi:10.1016/j.ab.2022.115018

Sample preparation for lipid analysis of intra-articular adipose tissue by using matrix-assisted laser desorption/ionization imaging

Anal Biochem. 2023 Feb 1:662:115018. doi: 10.1016/j.ab.2022.115018. Epub 2022 Dec 12.

Authors

Mirella J J Haartmans¹, Britt S R Claes², Kaj S Emanuel³, Gabrielle J M Tuijthof⁴, Ron M A Heeren⁵, Pieter J Emans⁶, Berta Cillero-Pastor⁷

Affiliations

¹ Maastricht MultiModal Molecular Imaging Institute (M4i), Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, the Netherlands; Laboratory for Experimental Orthopedics, Department of Orthopedic Surgery, Joint-Preserving Clinic, CAPHRI Care and Public Health Research Institute, Maastricht University Medical Center+, Maastricht, the Netherlands. Electronic address: m.haartmans@maastrichtuniversity.nl.
² Maastricht MultiModal Molecular Imaging Institute (M4i), Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, the Netherlands. Electronic address: britt.claes@maastrichtuniversity.nl.
³ Laboratory for Experimental Orthopedics, Department of Orthopedic Surgery, Joint-Preserving Clinic, CAPHRI Care and Public Health Research Institute, Maastricht University Medical Center+, Maastricht, the Netherlands; Amsterdam UMC, Amsterdam Movement Sciences, Department of Orthopedic Surgery and Sports Medicine, Amsterdam, the Netherlands. Electronic address: k.emanuel@maastrichtuniversity.nl.
⁴ Faculty of Engineering Technology, Biomedical Device Design and Production Technology (BDDP), Twente University, Twente, the Netherlands. Electronic address: g.j.m.tuijthof@utwente.nl.
⁵ Maastricht MultiModal Molecular Imaging Institute (M4i), Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, the Netherlands. Electronic address: r.heeren@maastrichtuniversity.nl.
⁶ Laboratory for Experimental Orthopedics, Department of Orthopedic Surgery, Joint-Preserving Clinic, CAPHRI Care and Public Health Research Institute, Maastricht University Medical Center+, Maastricht, the Netherlands. Electronic address: p.emans@mumc.nl.
⁷ Maastricht MultiModal Molecular Imaging Institute (M4i), Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, the Netherlands; MERLN Institute for Technology-inspired Regenerative Medicine, Department of Cell Biology-Inspired Tissue Engineering (cBITE), Maastricht University, Maastricht, the Netherlands. Electronic address: b.cilleropastor@maastrichtuniversity.nl.

PMID: 36521559
DOI: 10.1016/j.ab.2022.115018

Abstract

Mass spectrometry imaging (MSI) is a powerful technique enabling the visualization of the spatial distribution of different molecules in tissue biopsies with different pathologies. Sample handling and preparing adipose tissue for MSI is challenging and prone to molecular delocalization due to tissue melting. In this work, we developed a method for matrix-assisted laser desorption/ionization (MALDI)-MSI to study lipids in human infrapatellar fat pad (IPFP), a biomarker source in musculoskeletal pathologies, while preserving molecular spatial distribution. Cryosectioning at 15 μm with a temperature below -30 °C, thaw-mounting, and sublimation, was demonstrated to preserve IPFP's heterogeneous appearance and spatial distribution of lipids.

Keywords: Adipose tissue; Delocalization; Hoffa's fat pad; Lipidomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Diagnostic Imaging*
Humans
Lasers
Lipids / analysis
Specimen Handling*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods

Substances

Lipids