Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling

Yong Ge; Mojgan Zadeh; Mansour Mohamadzadeh

doi:10.1016/j.xpro.2022.101936

Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling

STAR Protoc. 2023 Mar 17;4(1):101936. doi: 10.1016/j.xpro.2022.101936. Epub 2022 Dec 13.

Authors

Yong Ge¹, Mojgan Zadeh¹, Mansour Mohamadzadeh²

Affiliations

¹ Department of Microbiology, Immunology & Molecular Genetics, University of Texas Health, San Antonio, TX, USA; Division of Gastroenterology & Nutrition, Department of Medicine, University of Texas Health, San Antonio, TX, USA.
² Department of Microbiology, Immunology & Molecular Genetics, University of Texas Health, San Antonio, TX, USA; Division of Gastroenterology & Nutrition, Department of Medicine, University of Texas Health, San Antonio, TX, USA. Electronic address: zadehm@uthscsa.edu.

Abstract

Intestinal epithelium is composed of several cell types, which can be dissociated but difficult to maintain high cell viability due to anoikis. Herein, we describe a step-by-step protocol for the isolation of highly viable intestinal epithelial cells using ethylenediaminetetraacetate acid and TrypLE Express, which can subsequently be employed for multi-omic analyses, including single-cell RNA sequencing. For complete details on the use and execution of this protocol, please refer to Ge et al. (2022).¹.

Keywords: Cell isolation; Flow Cytometry/Mass Cytometry; Sequencing; Single Cell.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Cell Survival
Epithelial Cells
Intestinal Mucosa
Intestines*
Mice
Multiomics*

Abstract

Publication types

MeSH terms

Grants and funding