Comparative expression analysis of TEADs and their splice variants in mouse embryonic stem cells

Gene Expr Patterns. 2023 Mar:47:119302. doi: 10.1016/j.gep.2022.119302. Epub 2022 Dec 11.

Abstract

Transcriptional enhanced associate domain (TEAD) transcription factors play important roles in embryonic stem cell (ESC) renewal and differentiation. Four TEAD transcription factors (Tead1, Tead2, Tead3 and Tead4) and their various splice variants have been discovered in mice, but the expression pattern of them during pluripotency state transition is unclear. Here, we investigated the expression of TEADs and their splice variants in mouse ESCs at different pluripotent/differentiating states and adult mouse tissues. Our results preliminarily revealed the diversity and heterogeneity of TEAD family, which is helpful for understanding their overlapping and distinctive functions. Furthermore, a novel splice variant of Tead1 was identified and named Tead1 isoform 4.

Keywords: Alternative splicing; Embryonic stem cells; Hippo pathway; TEAD.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing / genetics
  • Cell Differentiation* / genetics
  • Cell Proliferation / genetics
  • Cell Self Renewal* / genetics
  • Cells, Cultured
  • Gene Expression Profiling
  • Mouse Embryonic Stem Cells* / metabolism
  • RNA Isoforms / genetics
  • RNA Splicing / genetics
  • TEA Domain Transcription Factors* / genetics
  • TEA Domain Transcription Factors* / metabolism

Substances

  • RNA Isoforms
  • TEA Domain Transcription Factors
  • Tead1 protein, mouse
  • Tead2 protein, mouse
  • Tead3 protein, mouse
  • Tead4 protein, mouse