Methods to Assess the Role of PARPs in Regulating Mitochondrial Oxidative Function

Tünde Kovács; Boglárka Rauch; Edit Mikó; Péter Bai

doi:10.1007/978-1-0716-2891-1_14

Methods to Assess the Role of PARPs in Regulating Mitochondrial Oxidative Function

Methods Mol Biol. 2023:2609:227-249. doi: 10.1007/978-1-0716-2891-1_14.

Authors

Tünde Kovács¹, Boglárka Rauch¹, Edit Mikó¹, Péter Bai^{2

3

4

5}

Affiliations

¹ Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
² Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Debrecen, Hungary. baip@med.unideb.hu.
³ MTA-DE Lendület Laboratory of Cellular Metabolism, Debrecen, Hungary. baip@med.unideb.hu.
⁴ Research Center for Molecular Medicine, University of Debrecen, Debrecen, Hungary. baip@med.unideb.hu.
⁵ MTA-DE Cell Biology and Signaling Research Group, Debrecen, Hungary. baip@med.unideb.hu.

PMID: 36515839
DOI: 10.1007/978-1-0716-2891-1_14

Abstract

PARP enzymes are involved in metabolic regulation and impact on a plethora of cellular metabolic pathways, among them, mitochondrial oxidative metabolism. The detrimental effects of PARP1 overactivation upon oxidative stress on mitochondrial oxidative metabolism was discovered in 1998. Since then, there was an enormous blooming in the understanding of the interplay between PARPs and mitochondria. Mitochondrial activity can be assessed by a comprehensive set of methods that we aim to introduce here.

Keywords: ARTD; Differentiation; Flow cytometry; Mitochondria; Mitochondrial fission; Mitochondrial fusion; Mitochondrial morphology; Oximetry; PARP; Seahorse extracellular flux analyzer; Substrate preference; Δψ.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Respiration*
Mitochondria* / metabolism
Oxidation-Reduction
Oxidative Stress