Oxadiazole Schiff Base as Fe3+ Ion Chemosensor: "Turn-off" Fluorescent, Biological and Computational Studies

Abstract

Compound, (E)-5-(4-((thiophen-2-ylmethylene)amino)phenyl)-1,3,4-oxadiazole-2-thiol (3) was synthesized via condensation reaction of 5-(4-aminophenyl)-1,3,4-oxadiazole-2-thiol with thiophene-2-carbaldehyde in ethanol. For the synthesis and structural confirmation the FT-IR, ¹H, ¹³C-NMR, UV-visible spectroscopy, and mass spectrometry were carried out. The long-term stability of the probe (3) was validated by the experimental as well as theoretical studies. The sensing behaviour of the compound 3 was monitored with various metal ions (Ca²⁺, Cr³⁺, Fe³⁺, Co²⁺, Mg²⁺, Na⁺, Ni²⁺, K⁺) using UV- Vis. and fluorescence spectroscopy techniques by various methods (effect of pH and density functional theory) which showing the most potent sensing behaviour with iron. Job's plot analysis confirmed the binding stoichiometry ratio 1:1 of Fe³⁺ ion and compound 3. The limit of detection (LOD), the limit of quantification (LOQ), and association constant (K_a) were calculated as 0.113 µM, 0.375 µM, and 5.226 × 10⁵ respectively. The sensing behavior was further confirmed through spectroscopic techniques (FT-IR and ¹H-NMR) and DFT calculations. The intercalative mode of binding of oxadiazole derivative 3 with Ct-DNA was supported through UV-Vis spectroscopy, fluorescence spectroscopy, viscosity, cyclic voltammetry, and circular dichroism measurements. The binding constant, Gibb's free energy, and stern-volmer constant were find out as 1.24 × 10⁵, -29.057 kJ/mol, and 1.82 × 10⁵ respectively. The cleavage activity of pBR322 plasmid DNA was also observed at 3 × 10^-5 M concentration of compound 3. The computational binding score through molecular docking study was obtained as -7.4 kcal/mol. Additionally, the antifungal activity for compound 3 was also screened using broth dilution and disc diffusion method against C. albicans strain. The synthesized compound 3 showed good potential scavenging antioxidant activity against DPPH and H₂O₂ free radicals.

Keywords: Antifungal; Antioxidant activity; Chemosensor; DNA interaction; Molecular docking studies; Oxadiazole Schiff base derivative.