Isolation, characterization and genome analysis of an orphan phage FoX4 of the new Foxquatrovirus genus

BMC Microbiol. 2022 Dec 13;22(1):304. doi: 10.1186/s12866-022-02719-3.

Abstract

The growing interest in the therapeutic application of bacteriophages leads to a drastic increase in the number of sequenced genomes. Luckily, recent insights in phage taxonomy facilitate the classification of phages in a comprehensive and data-driven manner as recently proposed by the International Committee on Taxonomy of Viruses. In this research, we present the taxonomical classification of a novel, narrow host range Xanthomonas phage FoX4, isolated from a Brussels sprouts field in Belgium infested with Xanthomonas campestris pv. campestris. The phage has a limited ability to lyse a bacterial culture, yet adsorbs efficiently to its host. Based on its genome sequence and low similarity to previously described phages, the phage comprises the novel phage genus Foxquatrovirus.

Keywords: Bacteriophage; Genomics; Phage biocontrol; Xanthomonas campestris.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophages* / genetics
  • Genome, Viral
  • Host Specificity
  • Siphoviridae* / genetics
  • Xanthomonas campestris* / genetics