Traditional immunofluorescence (IF) imaging assays are limited to the detection of just a few markers due to spectral overlap of fluorescent emission bands. Furthermore, standard fluorescent imaging instruments have a dynamic range that is too narrow to capture the full range of expression values seen in biology, precluding the accurate quantification of single-cell target expression. Here we describe a protocol for detection and quantification of dozens of protein targets with single-cell quantitative precision using an iterative staining approach called ChipCytometry™.
Keywords: ChipCytometry™; Multiplexed Immunofluorescence; Single-cell proteomics; Spatial multiplexing.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.