Recent Advances in Chromogens for Immunohistochemistry

Abstract

Various staining strategies and color combinations have been developed to perform single and double immunohistochemical staining on biological samples. However, until recently, the lack of appropriate chromogen color combinations has severely limited many of these methods. Fortunately, this situation has dramatically improved with the introduction of new chromogens and methods of analysis. This article reviews recent trends in multicolor immunohistochemical staining methods that are finding broad applications in both research and clinical laboratories.

Keywords: 3,3’-Diaminobenzidine tetrahydrochloride (DAB); Chromogens; High Contrast DAB; Immunohistochemistry; PermaBlack HRP; PermaBlue; PermaBlue HRP; PermaGreen; PermaGreen HRP; PermaRed; PermaRed HRP; PermaYellow HRP; Stable DAB.