Polyurethane and cellulose acetate micro-nanofibers containing rosemary essential oil, and decorated with silver nanoparticles for wound healing application

Anjum Hamid Rather; Rumysa Saleem Khan; Taha Umair Wani; Muheeb Rafiq; Arvind H Jadhav; Puneethkumar M Srinivasappa; Abdalla Abdal-Hay; Phalisteen Sultan; Sami-Ullah Rather; Javier Macossay; Faheem A Sheikh

doi:10.1016/j.ijbiomac.2022.12.048

Polyurethane and cellulose acetate micro-nanofibers containing rosemary essential oil, and decorated with silver nanoparticles for wound healing application

Int J Biol Macromol. 2023 Jan 31:226:690-705. doi: 10.1016/j.ijbiomac.2022.12.048. Epub 2022 Dec 10.

Affiliations

¹ Nanostructured and Biomimetic Lab, Department of Nanotechnology, University of Kashmir Hazratbal, Srinagar 190006, Jammu and Kashmir, India.
² Centre for Nano and Material Science (CNMS), Jain University, Jain Global Campus, Bangalore 562112, Karnataka, India.
³ Department of Mechanical Engineering, Faculty of Engineering, South Valley University, Qena 83523, Egypt; The University of Queensland, School of Dentistry, Oral Health Centre Herston, 288 Herston Road, Herston, QLD 4006, Australia.
⁴ Department of Cellular and Molecular Biotechnology, CSIR-Indian Institute of Integrative Medicine, Sanantnagar, Srinagar 190005, Jammu and Kashmir, India.
⁵ Department of Chemical and Materials Engineering, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
⁶ Department of Chemistry, The University of Texas Rio Grande Valley, 1201 W. University Dr., Edinburg, TX 78539, United States of America.
⁷ Nanostructured and Biomimetic Lab, Department of Nanotechnology, University of Kashmir Hazratbal, Srinagar 190006, Jammu and Kashmir, India. Electronic address: faheemnt@uok.edu.in.

PMID: 36513179
DOI: 10.1016/j.ijbiomac.2022.12.048

Abstract

In this study, polyurethane (PU) and cellulose acetate (CA) electrospun fibers encapsulating rosemary essential oil (REO) and adsorbed silver (Ag) nanoparticles (NPs) were fabricated. The biologically inspired materials were analyzed for physicochemical characteristics using scanning electron microscopy, X-ray diffractometer, Fourier transform infrared, thermal gravimetric analysis, X-ray photoelectron spectroscopy, water contact angle, and water uptake studies. Results confirmed the presence of CA and Ag NPs on the PU micro-nanofibers increased the hydrophilicity from 107.1 ± 0.36^o to 26.35 ± 1.06^o. The water absorption potential increased from 0.07 ± 0.04 for pristine PU fibers to 12.43 ± 0.49 % for fibers with 7 wt% of CA, REO, and Ag NPs. The diffractometer confirmed the 2θ of 38.01°, 44.13^o, and 64.33^o, corresponding to the diffraction planes of Ag on the fibers. The X-ray photoelectron spectroscopy confirmed microfibers interfacial chemical interaction and surface changes due to CA, REO, and Ag presence. The inhibition tests on Staphylococcus aureus and Escherichia coli indicated that composites are antibacterial in activity. Moreover, synergistic interactions of REO and Ag NPs resulted in superior antibacterial activity. The cell viability and attachment assay showed improved hydrophilicity of the fibers, which resulted in better attachment of cells to the micro-nanofibers, similar to the natural extracellular matrix in the human body.

Keywords: Antibacterial mechanism; Cell viability; Tissue engineering.

MeSH terms

Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology
Humans
Metal Nanoparticles* / chemistry
Nanofibers* / chemistry
Oils, Volatile* / pharmacology
Polyurethanes
Rosmarinus*
Silver / chemistry
Wound Healing

Substances

Polyurethanes
Silver
acetylcellulose
Anti-Bacterial Agents
Oils, Volatile