Analysis of Golgi Morphology Using Immunofluorescence and CellProfiler Software

Isabel Mejia; Yu-Chuan Chen; Begoña Díaz

doi:10.1007/978-1-0716-2639-9_46

Analysis of Golgi Morphology Using Immunofluorescence and CellProfiler Software

Methods Mol Biol. 2023:2557:765-784. doi: 10.1007/978-1-0716-2639-9_46.

Authors

Isabel Mejia¹, Yu-Chuan Chen¹, Begoña Díaz^{2

3}

Affiliations

¹ Department of Internal Medicine, Division of Medical Hematology and Oncology, The Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA, USA.
² Department of Internal Medicine, Division of Medical Hematology and Oncology, The Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA, USA. bdiaz@lundquist.org.
³ David Geffen School of Medicine and Jonsson Comprehensive Cancer Center, University of California at Los Angeles, Los Angeles, CA, USA. bdiaz@lundquist.org.

PMID: 36512250
DOI: 10.1007/978-1-0716-2639-9_46

Abstract

The architecture of the Golgi apparatus in mammalian cells changes dynamically in response to internal and external cues and may be permanently altered in disease states. Here, we present a method to quantify changes in Golgi morphology using immunofluorescence and confocal microscopy followed by CellProfiler software analysis. This method will assist researchers in evaluating alterations in the Golgi complex morphology of cultured cells under a variety of different experimental conditions.

Keywords: GM130; Golgi area; Golgi compactness; Golgi fragmentation; Golgi morphology.

MeSH terms

Animals
Autoantigens*
Fluorescent Antibody Technique
Golgi Apparatus
Mammals
Membrane Proteins*
Software

Substances

Membrane Proteins
Autoantigens