Improved probe detection sensitivity by flexible tandem biotin labeling

Wenjie Cai; Mingqi Lu; Xiaoyan Cheng; Weijun Dai

doi:10.1016/j.mimet.2022.106654

Improved probe detection sensitivity by flexible tandem biotin labeling

J Microbiol Methods. 2023 Jan:204:106654. doi: 10.1016/j.mimet.2022.106654. Epub 2022 Dec 10.

Authors

Wenjie Cai¹, Mingqi Lu¹, Xiaoyan Cheng¹, Weijun Dai²

Affiliations

¹ Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China; College of Plant Protection, Integrative Microbiology Research Center, South China Agricultural University, Guangzhou 510642, China.
² Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China; College of Plant Protection, Integrative Microbiology Research Center, South China Agricultural University, Guangzhou 510642, China. Electronic address: daiweijun@scau.edu.cn.

PMID: 36509134
DOI: 10.1016/j.mimet.2022.106654

Abstract

We develop a biotin-based tandem labeling approach to improve detection sensitivity of DNA probe. Through DNA polymerase-mediated overhand filling, the 3'end of DNA probe was tandemly labeled with biotin molecules. The intensity of biotin signals could be flexibly manipulated by controlling the introduced length of poly(A) in the 5' overhang.

Keywords: Biotin; EMSA; Non-isotope; Probe labeling.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biotin*
Biotinylation
DNA Probes

Substances

Biotin
DNA Probes