Synthesis of siRNA-Conjugated Dextran-Coated Iron Oxide Nanoparticles for Islet Protection During Transplantation and Noninvasive Imaging

Neil Robertson; Ping Wang; Nazanin Talebloo; Kazuhiko Yamada; Anna Moore

doi:10.1007/978-1-0716-2807-2_11

Synthesis of siRNA-Conjugated Dextran-Coated Iron Oxide Nanoparticles for Islet Protection During Transplantation and Noninvasive Imaging

Methods Mol Biol. 2023:2592:163-174. doi: 10.1007/978-1-0716-2807-2_11.

Authors

Neil Robertson^{1

2}, Ping Wang^{1

2}, Nazanin Talebloo^{1

3}, Kazuhiko Yamada^{4

5}, Anna Moore^{6

7}

Affiliations

¹ Precision Health Program, Michigan State University, East Lansing, MI, USA.
² Department of Radiology, College of Human Medicine, Michigan State University, East Lansing, MI, USA.
³ Department of Chemistry, College of Natural Sciences, Michigan State University, East Lansing, MI, USA.
⁴ Columbia Center for Translational Immunology, Department of Surgery, Columbia University Irving Medical Center, New York, NY, USA.
⁵ Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA.
⁶ Precision Health Program, Michigan State University, East Lansing, MI, USA. moorea57@msu.edu.
⁷ Department of Radiology, College of Human Medicine, Michigan State University, East Lansing, MI, USA. moorea57@msu.edu.

PMID: 36507992
DOI: 10.1007/978-1-0716-2807-2_11

Abstract

Pancreatic islet transplantation (Tx) has a lifesaving potential for type 1 diabetes (T1D) patients. Islet damage during and after transplantation is one of the major reasons hampering its wide clinical application. Inability to monitor transplanted islets also severely limits our understanding of mechanisms regarding declining graft function after transplantation. Our team has proposed to use magnetic nanoparticles conjugated to siRNA (MN-siRNA) to label islets prior to transplantation with two goals in mind: to protect them from damage by silencing harmful genes and to monitor them after transplantation using noninvasive magnetic resonance imaging (MRI). This manuscript provides a step-by-step protocol for the synthesis and characterization of MN-siRNA probes.

Keywords: In vivo imaging; Iron oxide nanoparticles; Islet transplantation; Type I diabetes; siRNA.

MeSH terms

Dextrans
Humans
Islets of Langerhans Transplantation* / methods
Islets of Langerhans*
Magnetic Iron Oxide Nanoparticles
Magnetic Resonance Imaging / methods
RNA, Small Interfering / genetics

Substances

Dextrans
RNA, Small Interfering