Insecticide resistance status of malaria vectors in the malaria endemic states of India: implications and way forward for malaria elimination

Kamaraju Raghavendra; Manju Rahi; Vaishali Verma; Poonam Sharma Velamuri; Divya Kamaraju; Kalpana Baruah; Jyoti Chhibber-Goel; Amit Sharma

doi:10.1016/j.heliyon.2022.e11902

Insecticide resistance status of malaria vectors in the malaria endemic states of India: implications and way forward for malaria elimination

Heliyon. 2022 Nov 26;8(12):e11902. doi: 10.1016/j.heliyon.2022.e11902. eCollection 2022 Dec.

Authors

Kamaraju Raghavendra¹, Manju Rahi², Vaishali Verma¹, Poonam Sharma Velamuri¹, Divya Kamaraju², Kalpana Baruah³, Jyoti Chhibber-Goel⁴, Amit Sharma^{1

4}

Affiliations

¹ ICMR-National Institute of Malaria Research (NIMR), Sector 8, Dwarka, Delhi, India.
² Indian Council of Medical Research (ICMR), Ramalingaswami Bhavan, New Delhi, India.
³ National Vector Borne Disease Control Programme, Shastri Park, New Delhi, India.
⁴ Molecular Medicine, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, India.

Abstract

Background: In 2012, the World Health Organization (WHO) released the Global Plan for Insecticide Resistance Management in malaria vectors to stress the need to address insecticide resistance. In a prospective multi-centric study commissioned by the Indian Council of Medical Research (ICMR), we assessed the insecticide susceptibility status of the primary malaria vectors in India from 2017 through 2019.

Methods: The insecticide susceptibility status of the prevalent primary malaria vectors - An. culicifacies, An. fluviatilis, An. stephensi, An. minimus and An. baimaii and secondary malaria vectors - An. aconitus, An. annularis and An. philippinensis/nivepes from 328 villages in 79 districts of 15 states of India were assessed following the WHO method mainly to insecticides used in vector control, organochlorine (DDT), organophosphate (malathion), and other pyrethroids (alpha-cypermethrin, cyfluthrin, lambda-cyhalothrin and permethrin). The study sites were selected as suggested by the National Vector Borne Disease Control Programme.

Results: The primary malaria vector An. culicifacies showed resistance to DDT (50/50 districts including two districts of Northeastern India), malathion (27/44 districts), and deltamethrin (17/44 districts). This species was resistant to DDT alone in 19 districts, double resistant to DDT-malathion in 16 districts, double resistant to DDT-deltamethrin in 6 districts, and triple resistant to DDT-malathion-deltamethrin in 9 districts. An. minimus and An. baimaii were susceptible in Northeastern India while An. fluviatilis and the secondary malaria vector An. annularis was resistant to DDT in Jharkhand.

Conclusion: In this study we report that among the primary vectors An. culicifacies is predominantly resistant to multiple insecticides. Our data suggest that periodic monitoring of insecticide susceptibility is vital. The national malaria program can take proactive steps for insecticide resistance management to continue its push toward malaria elimination in India.

Keywords: Anopheles; India; Insecticide-resistance; Malaria vectors.