Brain organoids: Establishment and application

Hao Chen; Xin Jin; Tie Li; Zhuang Ye

doi:10.3389/fcell.2022.1029873

Brain organoids: Establishment and application

Front Cell Dev Biol. 2022 Nov 23:10:1029873. doi: 10.3389/fcell.2022.1029873. eCollection 2022.

Authors

Hao Chen¹, Xin Jin², Tie Li³, Zhuang Ye³

Affiliations

¹ Department of Neurovascular Surgery, First Hospital, Jilin University, Changchun, China.
² Department of Oncology and Hematology, Second Hospital, Jilin University, Changchun, China.
³ Department of Rheumatology, First Hospital, Jilin University, Changchun, China.

Abstract

Brain organoids are produced by the differentiation of pluripotent stem cells under three-dimensional culture conditions by adding neurodevelopment-related regulatory signals. They are similar to the cell composition and anatomical structure of the brain, and can reflect the developmental process of the brain, as well as their physiology, pathology, and pharmacology. Brain organoids are good models to study human brain development and brain-related diseases in vitro. Here, we mainly focus on the construction of brain organoids and review the application of brain organoids in disease modelingand drug screening.

Keywords: 3D culture; brain disease modeling; brain organoids; drug screening; neurological diseases; organ transplantation; organoid; pluripotent stem cells.

Publication types

Review