Non-photosynthetic chemoautotrophic CO2 assimilation microorganisms carbon fixation efficiency and control factors in deep-sea hydrothermal vent

Chenglong Xu; Yali Feng; Haoran Li; Shiwei Jiang; Ruiyu Ma; Yisong Yao; Mengyao Liu; Yi Yang; Zhonghua Xue

doi:10.1016/j.scitotenv.2022.160805

Non-photosynthetic chemoautotrophic CO₂ assimilation microorganisms carbon fixation efficiency and control factors in deep-sea hydrothermal vent

Sci Total Environ. 2023 Mar 1:862:160805. doi: 10.1016/j.scitotenv.2022.160805. Epub 2022 Dec 8.

Authors

Chenglong Xu¹, Yali Feng², Haoran Li³, Shiwei Jiang⁴, Ruiyu Ma⁵, Yisong Yao⁴, Mengyao Liu⁴, Yi Yang⁶, Zhonghua Xue⁴

Affiliations

¹ School of Civil and Resource Engineering, University of Science and Technology Beijing, Beijing 100083, China; State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
² School of Civil and Resource Engineering, University of Science and Technology Beijing, Beijing 100083, China. Electronic address: ylfeng126@126.com.
³ State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: hrli@ipe.ac.cn.
⁴ School of Civil and Resource Engineering, University of Science and Technology Beijing, Beijing 100083, China.
⁵ School of Civil and Resource Engineering, University of Science and Technology Beijing, Beijing 100083, China; State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
⁶ State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.

PMID: 36502982
DOI: 10.1016/j.scitotenv.2022.160805

Abstract

Non-photosynthetic chemoautotrophic microorganisms in deep-sea hydrothermal vent can obtain energy by oxidation reducing substances and synthesize CO₂ into organic carbon, and the development and utilization of microbial resources in this environment for CO₂ fixation under ordinary environmental conditions is of great significance to understand the carbon cycle and microbial carbon fixation in deep-sea hydrothermal vent. In this study, a set of spiral-stirred bioreactor (SSB) was developed to cultivate a group of non-photosynthetic chemoautotrophic CO₂ assimilation microorganisms (NPCAM), mainly Sphingomonadaceae (unclassified, the mean of which was 31.16 %), from deep-sea hydrothermal vent sediments, which have the characteristics of halophilic, acid-base and heavy metal resistant. The maximum carbon fixation efficiency (calculated by CO₂) was 6.209 mg·CO₂/(L·h) after 96 h of incubation in the presence of mixed electron donors (MEDs, 0.46 % NaNO₂, 0.50 % Na₂S₂O₃ and 1.25 % Na₂S, w/v), mixed inorganic carbon sources (CO₂, Na₂CO₃ and NaHCO₃) and aerobic conditions. The detection of NPCAM synthetic organic fraction in SSB system, the study of single bacteria culturability and carbon fixation efficiency, the analysis of CO₂ fixation pathway and the development of coupled carbon fixation technology are the prospective works that need to be further developed.

Keywords: Deep-sea hydrothermal vent; Electron donors; Environmental factors; Inorganic carbon sources; Non-photosynthetic chemoautotrophic CO(2) assimilation microorganisms; Spiral-stirred bioreactor.

MeSH terms

Carbon / metabolism
Carbon Cycle
Carbon Dioxide* / metabolism
Hydrothermal Vents* / microbiology
Prospective Studies

Substances

Carbon Dioxide
N-(1-pyrenyl)iodoacetamide
Carbon