Development of a responsive probe for colorimetric and fluorescent detection of bisulfite in food and animal serum samples in 100% aqueous solution

Yifang Li; Yao Wang; Xiaoman Lei; Kaitong Guo; Qian Ai; Feifan Zhang; Xiujin Chen; Xiaofei Sun; Tong-Tong Jia; Yashan Li; Huawei Niu; Yong Ye

doi:10.1016/j.foodchem.2022.135146

Development of a responsive probe for colorimetric and fluorescent detection of bisulfite in food and animal serum samples in 100% aqueous solution

Food Chem. 2023 May 1:407:135146. doi: 10.1016/j.foodchem.2022.135146. Epub 2022 Dec 5.

Authors

Yifang Li¹, Yao Wang², Xiaoman Lei¹, Kaitong Guo¹, Qian Ai¹, Feifan Zhang¹, Xiujin Chen¹, Xiaofei Sun¹, Tong-Tong Jia³, Yashan Li⁴, Huawei Niu⁵, Yong Ye⁶

Affiliations

¹ College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, PR China.
² College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, PR China. Electronic address: tiger86121@163.com.
³ College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang 471022, PR China.
⁴ College of Resources, Environment and Chemistry, Chuxiong Normal University, Chuxiong 675000, PR China. Electronic address: liyashan@cxtc.edu.cn.
⁵ College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, PR China. Electronic address: niuhw0816@126.com.
⁶ Green Catalysis Center, and College of Chemistry, Zhengzhou University, Zhengzhou 450001, PR China.

PMID: 36502733
DOI: 10.1016/j.foodchem.2022.135146

Abstract

Bisulfite (HSO₃^-) has the functions of bleaching, antiseptic, antioxidant, inhibiting bacterial growth, and controlling enzymatic reactions in food. However, long-term consumption of foods containing excessive amounts of bisulfite can be harmful to health. In addition, large doses of sulfur dioxide (SO₂) can cause diarrhea, hypotension, allergic and asthmatic reactions in susceptible individuals. Therefore, it is urgent and essential to explore some rapid, reliable, and convenient tools to detect HSO₃^- in food and SO₂ gas. Herein, we exploited a fluorescent probe, NPO, to detect HSO₃^- in 100 % aqueous solution. The probe has the advantages of easy synthesis, excellent water solubility, significant colorimetric change, good selectivity, high sensitivity, and fast response (within 1 min). Probe NPO was successfully applied for testing strips to visualize the behavior of HSO₃^- and SO₂ gas. Moreover, the probe has been used to monitor the behavior of HSO₃^- in real food samples and animal serum samples.

Keywords: Animal serum samples; Colorimetric; Fluorescence probe; HSO(3)(−); Real food samples; SO(2) gas.

MeSH terms

Animals
Colorimetry*
Fluorescent Dyes
Sulfites
Sulfur Dioxide
Water*

Substances

hydrogen sulfite
Water
Fluorescent Dyes
Sulfites
Sulfur Dioxide