Multiplex PCR for detection of acquired plasmid-borne fosfomycin resistance fos genes in Escherichia coli

Diagn Microbiol Infect Dis. 2023 Feb;105(2):115864. doi: 10.1016/j.diagmicrobio.2022.115864. Epub 2022 Nov 23.

Abstract

A rapid (<3 hours) and reliable multiplex PCR was developed for detecting simultaneously known plasmid-mediated fos genes conferring acquired resistance to fosfomycin. Our technique was tested on a collection of Escherichia coli isolates previously identified as bearing the fosA-, fosC- and fosL-like genes, showing a sensitivity and a specificity of 100%.

Keywords: Escherichia coli; fosA; fosC; fosL; fosfomycin resistance; multiplex PCR.

MeSH terms

  • Anti-Bacterial Agents* / pharmacology
  • Drug Resistance, Bacterial* / genetics
  • Escherichia coli Infections* / diagnosis
  • Escherichia coli* / drug effects
  • Escherichia coli* / genetics
  • Escherichia coli* / isolation & purification
  • Fosfomycin* / pharmacology
  • Humans
  • Microbial Sensitivity Tests
  • Multiplex Polymerase Chain Reaction*
  • Plasmids / genetics
  • beta-Lactamases / genetics

Substances

  • Anti-Bacterial Agents
  • beta-Lactamases
  • Fosfomycin