Transcriptional Responses for Biosynthesis of Triterpenoids in Exogenous Inducers Treated Inonotus Hispidus Using RNA-Seq

Yonghong Huo; Dongchao Liu; Qin Yang; Changyan Sun; Zhanbin Wang; Dehai Li

doi:10.3390/molecules27238541

Transcriptional Responses for Biosynthesis of Triterpenoids in Exogenous Inducers Treated Inonotus Hispidus Using RNA-Seq

Molecules. 2022 Dec 4;27(23):8541. doi: 10.3390/molecules27238541.

Authors

Yonghong Huo¹, Dongchao Liu¹, Qin Yang¹, Changyan Sun², Zhanbin Wang¹, Dehai Li¹

Affiliations

¹ School of Forestry, Northeast Forestry University, Harbin 150040, China.
² Department of Environmental Engineering, School of Materials Science and Chemical Engineering, Harbin University of Science and Technology, Harbin 150040, China.

Abstract

Inonotus hispidus is a traditional medicinal that grows in Northeast China and produces various economically important compounds, including polysaccharide compounds and terpenoids; triterpenoid saponins is the main bioactive component. Our research group has found that the accumulation of triterpenoid was affected by exogenous inducers. Experimental results showed that treatment with methyl jasmonate (MeJA) and oleic acid significantly increased the triterpenoid content of I. hispidus. However, how exogenous inducers enhance production of secondary metabolites in I. hispidus is not well understood. In this study, metabolite changes were further investigated with UPLC-TOF/MS following exogenous inducer treatment. As a result, a total of eight types of triterpenoids in I. hispidus were identified. The RNA-seq analysis was used to evaluate the effects of exogenous inducers on the expression of triterpenoid-synthesis-related genes in I. hispidus in liquid fermentation. This study is the first exploration to profile the transcriptome of I. hispidus after adding exogenous inducers; the generated data and gene will facilitate further molecular studies on the physiology and metabolism in this fungi. By comparative transcriptomic analysis, a series of candidate genes involved in the biosynthetic pathway of triterpenoids are identified, providing new insights into their biosynthesis at the transcriptome level.

Keywords: Inonotus hispidus; RNA-Seq; differential expressed genes; exogenous inducer.

MeSH terms

Biosynthetic Pathways / genetics
Gene Expression Profiling
RNA-Seq
Transcriptome
Triterpenes* / pharmacology

Substances

Triterpenes

Grants and funding

This research was funded by the Natural Science Foundation of Heilongjiang Province [LH2022C007], the Hundred Million Science and Technology Major Special Project of Heilongjiang Province [2020ZX07B01-3], Harbin Science and Technology Bureau Science and Technology Innovation Talent Research Fund [2017RAQXJ091], and the China Scholarship Commission. We are very grateful to Ultra high Performance Liquid Chromatography Tandem time of Flight Mass Spectrometry (UPLC-TOF-MS/MS),Facility at Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University.