Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes

Mst Ayesha Siddika; Takahiro Yamada; Risako Aoyama; Kumi Hidaka; Hiroshi Sugiyama; Masayuki Endo; Shigeyoshi Matsumura; Yoshiya Ikawa

doi:10.3390/molecules27238298

Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes

Molecules. 2022 Nov 28;27(23):8298. doi: 10.3390/molecules27238298.

Authors

Mst Ayesha Siddika¹, Takahiro Yamada², Risako Aoyama², Kumi Hidaka³, Hiroshi Sugiyama³, Masayuki Endo^{3

4}, Shigeyoshi Matsumura^{1

2}, Yoshiya Ikawa^{1

2}

Affiliations

¹ Graduate School of Innovative Life Science, University of Toyama, Gofuku 3190, Toyama 930-8555, Japan.
² Department of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama 930-8555, Japan.
³ Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto 606-8501, Japan.
⁴ Organization for Research and Development of Innovative Science and Technology, Kansai University, Osaka 564-8680, Japan.

Abstract

Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner. In this study, we designed and constructed open-form oligomers of a bimolecular form of an RNase P ribozyme. The ribozyme oligomers were analyzed biochemically and by atomic force microscopy (AFM).

Keywords: RNA motif; RNA nanostructure; pre-tRNA processing; ribonuclease P; ribozyme.

MeSH terms

Microscopy, Atomic Force
Nucleic Acid Conformation
RNA / chemistry
RNA / genetics
RNA, Catalytic* / chemistry
Ribonuclease P / genetics

Substances

RNA, Catalytic
Ribonuclease P
RNA

Grants and funding

2022/University of Toyama Discretionary Funds of the President