Extraction and characterization of hyaluronic acid from the eyeball of Nile Tilapia (Oreochromis niloticus)

Lyndervan Oliveira Alcântara; Juliana Rabelo de Sousa; Fábia Karine Andrade; Edson Holanda Teixeira; Miguel Ângelo Cerqueira; André Luis Coelho da Silva; Men de Sá Moreira Souza Filho; Bartolomeu Warlene Silva de Souza

doi:10.1016/j.ijbiomac.2022.12.016

Extraction and characterization of hyaluronic acid from the eyeball of Nile Tilapia (Oreochromis niloticus)

Int J Biol Macromol. 2023 Jan 31:226:172-183. doi: 10.1016/j.ijbiomac.2022.12.016. Epub 2022 Dec 7.

Authors

Lyndervan Oliveira Alcântara¹, Juliana Rabelo de Sousa¹, Fábia Karine Andrade², Edson Holanda Teixeira³, Miguel Ângelo Cerqueira⁴, André Luis Coelho da Silva⁵, Men de Sá Moreira Souza Filho⁶, Bartolomeu Warlene Silva de Souza⁷

Affiliations

¹ Department of Fishing Engineering, Federal University of Ceara, Campus do Pici, 825, CEP: 60356-000 Fortaleza, CE, Brazil.
² Department of Chemical Engineering, Graduate Program of Chemical Engineering, Federal University of Ceara, Campus do Pici, 709, CEP: 60455-760 Fortaleza, CE, Brazil.
³ Department of Pathology and Forensic Medicine, Faculty of Medicine, Federal University of Ceara, UFC, CEP: 60430-160 Fortaleza, CE, Brazil.
⁴ International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga s/n, 4715-330 Braga, Portugal.
⁵ Department of Biochemistry and Molecular Biology, Federal University of Ceara, Campus do Pici, 907 CEP: 60451-970, Fortaleza, CE, Brazil.
⁶ Embrapa Tropical Agroindustry, Rua Dr. Sara Mesquita, 2270, Planalto Pici, CEP: 60511-110 Fortaleza, CE, Brazil.
⁷ Department of Fishing Engineering, Federal University of Ceara, Campus do Pici, 825, CEP: 60356-000 Fortaleza, CE, Brazil. Electronic address: souzabw@ufc.br.

PMID: 36495987
DOI: 10.1016/j.ijbiomac.2022.12.016

Abstract

Hyaluronic acid (HA) is a biopolymer of enormous value aggregation for in general industry. The vitreous humor of the eyeball from Nile tilapia contains appreciable amounts of hyaluronic acid. In this sense, the aim of this work was to extract and characterize hyaluronic acid from the eyeball of the Nile tilapia for biomedical applications, adding value to fish industry residues. The characterization by infra-red (FTIR), ¹³C nuclear magnetic resonance (NMR) and high performance liquid chromatography (HPLC) confirmed that hyaluronic acid was obtained. The gel permeation chromatography (GPC) showed that the obtained material presents a low molecular mass (37 KDa). Thermogravimetry (TGA), differential scanning calorimetry (DSC), X-ray diffraction (XRD) analysis showed that the materials present a thermal stability superior to the commercial hyaluronic acid from Streptococcus equi, with a partially crystalline character. The cytotoxicity assay (MTT method) with fibroblast cells (L929) demonstrated that the extracted biopolymer besides not being cytotoxic, was able to stimulate cell proliferation. Therefore, the hyaluronic acid extracted from this source of residue constitutes a product with biotechnological potential, which has adequate quality for wide biomedical applications.

Keywords: Eyeball; Hyaluronic acid; Oreochromis niloticus; Value aggregation.

MeSH terms

Animals
Cichlids*
Fish Diseases*
Hyaluronic Acid

Substances

Hyaluronic Acid