Development of a phage-based electrochemical biosensor for detection of Escherichia coli O157: H7 GXEC-N07

Yuqing Zhou; Ziyong Li; Jijie Huang; Yuxing Wu; Xinyu Mao; Yizhou Tan; Hui Liu; Dongxin Ma; Xun Li; Xiaoye Wang

doi:10.1016/j.bioelechem.2022.108345

Development of a phage-based electrochemical biosensor for detection of Escherichia coli O157: H7 GXEC-N07

Bioelectrochemistry. 2023 Apr:150:108345. doi: 10.1016/j.bioelechem.2022.108345. Epub 2022 Dec 5.

Authors

Yuqing Zhou¹, Ziyong Li¹, Jijie Huang¹, Yuxing Wu¹, Xinyu Mao¹, Yizhou Tan¹, Hui Liu¹, Dongxin Ma¹, Xun Li², Xiaoye Wang³

Affiliations

¹ College of Animal Science and Technology, Guangxi University, Nanning 530004, Guangxi, China.
² College of Animal Science and Technology, Guangxi University, Nanning 530004, Guangxi, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning 530004, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning 530004, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, Nanning 530004, China. Electronic address: xunli@gxu.edu.cn.
³ College of Animal Science and Technology, Guangxi University, Nanning 530004, Guangxi, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning 530004, China; Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning 530004, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control for Animal Disease, Nanning 530004, China. Electronic address: xywang@gxu.edu.cn.

PMID: 36495704
DOI: 10.1016/j.bioelechem.2022.108345

Abstract

Escherichia coli (E. coli) O157:H7 is one of the most important foodborne pathogens that causing severe foodborne diseases. With the development of foodborne diseases, there is an urgent need to seek new methods for early detection and monitoring of E. coli O157:H7. In this study, an electrochemical biosensor using phage EP01 as the recognition agent for detection of E. coli O157:H7 GXEC-N07 was established due to the specificity and high efficiency of phage EP01 in recognizing GXEC-N07. The biosensor was developed by depositing phages conjugated carboxyl graphene oxide (CFGO) and conductive carbon black (CB) onto the surface of glass carbon electrodes (GCEs). When detecting GXEC-N07 in the concentration range of 10² ∼ 10⁷ CFU/mL, the biosensor showed good linearity with a low detection limit of 11.8 CFU/mL, and the whole progress was in less than 30 min. The biosensor was successfully applied to the quantitative detection of GXEC-N07 in fresh milk and raw pork. The recovery values ranged from 60.8 % to 114.2 %. The biosensor provides a rapid, specific, low cost, and label free tool for E. coli O157:H7 GXEC-N07 detection. It is expected to become a powerful method for the detection of bacteria that threatening food safety and public health security.

Keywords: Bacterial detection; Bacteriophage; Electrochemical biosensor; Escherichia coli O157:H7; Foodborne pathogen.

MeSH terms

Bacteriophages*
Biosensing Techniques* / methods
Escherichia coli O157*
Food Contamination / analysis
Food Microbiology
Foodborne Diseases* / microbiology
Humans