An important point of the analysis of a single-cell RNA experiment is the identification of the key elements, i.e., genes, characterizing each cell subpopulation cluster. In this chapter, we describe the use of sparsely connected autoencoder, as a tool to convert single-cell clusters in pseudo-RNAseq experiments to be used as input for differential expression analysis, and the use of COMET, as a tool to depict cluster-specific gene markers.
Keywords: Comet; Feature selection; Sparsely connected autoencoder.
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