Electrokinetic sample manipulation is a key step for many kinds of microfluidic chips to achieve various functions, such as particle focusing and separation, fluid pumping and material synthesis. But these microfluidic experiments usually rely on large-scale signal generators for power supply, microscopes for imaging and other instruments for analysis, which hampers the portable process of microfluidic technology. Inspired by this situation, we herein designed a portable general microfluidic device (PGMD) with complex electric field regulation functions, which can accurately regulate static or continuous fluid samples. Through the graphical user interface (GUI) and modular design, the PGMD can generate multiple different electrical signals, and the micro-flow of fluid can be pumped through the built-in micropump, which can meet the requirements of most microfluidic experiments. Photos or videos of the microfluidic chip captured by the built-in microscope are received and displayed by a smartphone. We carried out a variety of microfluidic experiments such as induced-charge electroosmosis (ICEO), particle beam exit switching, thermal buoyancy flow and dielectrophoresis (DEP) on the PGMD. In addition, the PGMD can perform rapid microalgae concentration estimation in an outdoor environment, which can be used to guide microalgae cultivation, further demonstrating the development potential of this device in the field of microbial applications. Numerous results show that the PGMD has a high degree of integration and strong reliability, which expands the application of microfluidic electrokinetic experiments and provides technical support for the integration and portability of microfluidic experimental devices.