MicroRNA-455-3p accelerate malignant progression of tumor by targeting H2AFZ in colorectal cancer

Lizhen Ye; Tingting Fan; Ying Qin; Cheng Qiu; Lulu Li; Mengmeng Dai; Yaoyao Zhou; Yan Chen; Yuyang Jiang

doi:10.1080/15384101.2022.2154549

MicroRNA-455-3p accelerate malignant progression of tumor by targeting H2AFZ in colorectal cancer

Cell Cycle. 2023 Apr;22(7):777-795. doi: 10.1080/15384101.2022.2154549. Epub 2022 Dec 8.

Authors

Lizhen Ye¹, Tingting Fan², Ying Qin³, Cheng Qiu⁴, Lulu Li¹, Mengmeng Dai¹, Yaoyao Zhou¹, Yan Chen¹, Yuyang Jiang^{1

2

5}

Affiliations

¹ State Key Laboratory of Chemical Oncogenomics, Key Laboratory of Chemical Biology, Tsinghua Shenzhen International Graduate School, Shenzhen, Guangdong, China.
² Department of Chemistry, Southern University of Science and Technology, Shenzhen, China.
³ Department of Gastrointestinal Surgery, Shenzhen Second People's Hospital, Shenzhen, Guangdong, China.
⁴ National & Local United Engineering Lab for Personalized Anti-tumor Drugs, Shenzhen Kivita Innovative Drug Discovery Institute, Tsinghua Shenzhen International Graduate School, Shenzhen, Guangdong, China.
⁵ School of Pharmaceutical Sciences, Tsinghua University, Beijing, China.

Abstract

Colorectal cancer (CRC) becomes the second leading cause of cancer-related deaths in 2020. Emerging studies have indicated that microRNAs (miRNAs) play a key role in tumorigenesis and progression. The dysfunctions of miR-455-3p are observed in many cancers. However, its biological function in CRC remains to be confirmed. By sequencing serum sample, miR-455-3p was found to be up-regulated in CRC patients. RT-qPCR demonstrated that the miR-455-3p expression was both higher in the serum and tumor tissues of CRC patients. Furthermore, it indicated that miR-455-3p had the ability in promoting cell proliferation, suppressing cell apoptosis, and stimulating cell migration. In vivo experiments also showed that miR-455-3p promoted tumor growth. Additionally, H2AFZ was proved as the direct gene target of miR-455-3p by dual-luciferase assay. Taken together, miR-455-3p functioned as a tumor promoter in CRC development by regulating H2AFZ directly. Thus, it has enormous potential as a biomarker in the diagnosis of CRC.

Keywords: CRC; H2AFZ; miR-455-3p; tumor promoter.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis / genetics
Carcinogenesis / genetics
Cell Line, Tumor
Cell Movement / genetics
Cell Proliferation / genetics
Colorectal Neoplasms* / pathology
Gene Expression Regulation, Neoplastic
Humans
MicroRNAs* / genetics
MicroRNAs* / metabolism

Substances

MicroRNAs
MIRN455 microRNA, human

Grants and funding

The work was supported by the Department of Science and Technology of Guangdong Province [No. 2017B030314083]; Shenzhen Progression and Reform Committee [No.2019156].