Environmental surveillance can be a complementary tool for detecting pathogens circulating in communities. We detected monkeypox virus DNA in wastewater from Italy's largest airport by using real-time PCR assays targeting the G2R region and F3L and N3R genes and sequencing. Wastewater surveillance can be quickly adapted to investigate emerging threats.
Keywords: Italy; Monkeypox; Rome; airport; environmental surveillance; transportation hub; viruses; wastewater; zoonoses.