OMIP-087: Thirty-two parameter mass cytometry panel to assess human CD4 and CD8 T cell activation, memory subsets, and helper subsets

Alexis Sponaugle; Maria Abad-Fernandez; Nilu Goonetilleke

doi:10.1002/cyto.a.24707

OMIP-087: Thirty-two parameter mass cytometry panel to assess human CD4 and CD8 T cell activation, memory subsets, and helper subsets

Cytometry A. 2023 Mar;103(3):184-188. doi: 10.1002/cyto.a.24707. Epub 2022 Dec 5.

Authors

Alexis Sponaugle¹, Maria Abad-Fernandez², Nilu Goonetilleke^{1

2}

Affiliations

¹ Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
² Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States.

PMID: 36470845
DOI: 10.1002/cyto.a.24707

Abstract

We developed a highly reproducible 32-marker mass cytometry panel able to measure all canonical immune lineages and perform detailed characterization of both CD4 and CD8 T cells in human peripheral blood mononuclear cells. In this panel, we identify six different T cell memory subsets, as well as markers of activation, cell cycling, and survival. In addition, this panel classifies all major CD4 T cell helper subsets. This panel enables detailed monitoring of CD4 and CD8 T cells in the context of infectious disease, cancer or autoimmunity with limited patient sample use. Detailed methods for standardization and optimization of the panel can be found in Supporting Information.

Keywords: CD4 T cells; CD8 T cells; mass cytometry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes*
Flow Cytometry / methods
Humans
Leukocytes, Mononuclear*
Lymphocyte Activation
T-Lymphocyte Subsets
T-Lymphocytes, Helper-Inducer