Calcium ion removal at different sodium chloride concentrations by free and immobilized halophilic bacteria

Abstract

Much attention has been paid to Ca²⁺ ion removal by biomineralization due to the dangers of Ca²⁺ on industrial processes and human health. However, Ca²⁺ removal from hypersaline water by biomineralization is quite difficult due to there being few halophilic bacteria tolerating higher salinities. In this study, free and immobilized Virgibacillus massiliensis C halophilic bacteria exhibiting carbonic anhydrase activity were used to remove Ca²⁺ ions from water at different NaCl concentrations. With increasing NaCl concentrations (10, 50, 100, 150 and 200 g/L), Ca²⁺ ion concentrations in the presence of free bacteria and in two groups of immobilized bacteria for a period of 6 days sharply decreased from 1200 mg/L to 219-562 mg/L, 71-214 mg/L and 21-159 mg/L, respectively; Ca²⁺ precipitation ratios were 55%-81%, 82%-94% and 87%-98%, respectively. The humic acid-like substances, protein, DNA and polysaccharide, released by the bacteria, promoted the Ca²⁺ ion removal. The immobilized bacteria were able to be recycled and precultured, which would save industry costs and increase Ca²⁺ ion removal efficiency. Biological processes for Ca²⁺ ion removal include cell surface, intracellular and extracellular biomineralization. The biogenesis of calcium carbonate was proved by SEM-EDS, FTIR, XPS and stable carbon isotope values. This study provides insights into the effective removal of Ca²⁺ ions by biomineralization in hypersaline water.

Keywords: Biomineralization; Calcium removal; Higher NaCl concentrations; Precipitation ratio; Softening of hypersaline water; Virgibacillus massiliensis.