Anti-neuroinflammatory effects of Cleistocalyx nervosum var. paniala berry-seed extract in BV-2 microglial cells via inhibition of MAPKs/NF-κB signaling pathway

Sakawrat Janpaijit; Pattawika Lertpatipanpong; Chanin Sillapachaiyaporn; Seung Joon Baek; Somsri Charoenkiatkul; Tewin Tencomnao; Monruedee Sukprasansap

doi:10.1016/j.heliyon.2022.e11869

Anti-neuroinflammatory effects of Cleistocalyx nervosum var. paniala berry-seed extract in BV-2 microglial cells via inhibition of MAPKs/NF-κB signaling pathway

Heliyon. 2022 Nov 28;8(11):e11869. doi: 10.1016/j.heliyon.2022.e11869. eCollection 2022 Nov.

Authors

Sakawrat Janpaijit¹, Pattawika Lertpatipanpong², Chanin Sillapachaiyaporn¹, Seung Joon Baek², Somsri Charoenkiatkul³, Tewin Tencomnao^{4

5}, Monruedee Sukprasansap⁶

Affiliations

¹ Ph.D Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
² Laboratory of Signal Transduction, Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, South Korea.
³ Institute of Nutrition, Mahidol University, Salaya Campus, Phutthamonthon, Nakhonpathom, 73170, Thailand.
⁴ Natural Products for Neuroprotection and Anti-Ageing Research Unit, Chulalongkorn University, Bangkok 10330, Thailand.
⁵ Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
⁶ Food Toxicology Unit, Institute of Nutrition, Mahidol University, Salaya Campus, Phutthamonthon, Nakhonpathom, 73170, Thailand.

Abstract

Neuroinflammation is an essential contributor to multiple neurodegenerative disorders. Cleistocalyx nervosum var. paniala, an edible berry, has been reported to exhibit a neuroprotective effect. However, only limited research is available on this fruit seed, which is classified as agricultural food waste. We therefore focused on the anti-neuroinflammatory effects and mechanisms of C. nervosum var. paniala seed extract (CNSE) on lipopolysaccharide (LPS)-induced inflammatory response in BV-2 mouse microglial cells. HPLC analysis showed that CNSE consists of resveratrol (RESV). For cell-based studies, BV-2 cells were pre-treated with CNSE or RESV, followed by LPS. We found that CNSE and RESV inhibited LPS-induced inflammation in a dose-dependent manner. CNSE and RESV inhibited gene expression and activity of iNOS, leading to a decrease in nitric oxide production. Both CNSE and RESV suppressed the gene expression and the activities of TNF-α, IL-1β, and IL-6. Our results revealed that LPS stimulated the protein levels of MAPKs (JNK, ERK1/2, and p38), while pretreatment of cells with CNSE or RESV attenuated these proteins expressions. CNSE also suppressed NF-κB activation. These results suggest that CNSE and RESV can inhibit LPS-induced inflammatory response through MAPKs/NF-κB pathways in BV-2 cells. Taken together, CNSE have potential as a functional anti-neuroinflammatory agent.

Keywords: Lipopolysaccharide; MAPKs; Microglial cells; NF-κB; Neuroinflammation.