Reducing plant pathogens could increase crop yields after plastic film mulching

Abstract

Soil fungi are closely associated with crop growth in agricultural ecosystems through processes such as nutrient uptake and pathogenesis. Plastic film mulching (PM) plays a dominant role in increasing crop yields in dryland agriculture worldwide. The functional guilds of soil fungi under PM and their effects on crops remain unclear. In this study, we explored the absolute abundance, diversity, community composition, and functional guilds of soil fungi after short-term (2 years) and long-term (10 years) mulching experiments. Short-term mulching caused a 37 %-51 % decrease in absolute fungal abundance owing to abrupt changes in the microenvironment. The response of the fungal community to PM varied with sites, with the effect being more pronounced under poor hydrothermal conditions (314 mm). The abundance of potential fungal pathogens decreased under PM; for example, Gibberella (maize ear rot) abundance was 45 % and 72 % lower under short- and long-term mulching, respectively, when compared with that in control. In contrast, the abundance of plant biocontrol fungi increased under PM; for instance, Glomeromycota abundance increased twofold under long-term mulching. Although PM did not alter the complexity and stability of fungal co-occurrence network, competition among fungi increased in the absence of sufficient carbon (C) sources. Long-term mulching reduced phytopathogen guilds by 12 %-77 % and increased arbuscular mycorrhizal fungi (AMF) guilds by 89 %-94 %. Structural equation modeling suggested that PM altered fungal functional guilds mainly by shaping the structure of the fungal community, and fungal pathogens decreased with increased AMF functional guilds, inducing higher maize yields. These results showed for the first time, from a microbial perspective, that pathogens reduction owing to PM could explain 4.4 % of maize yield variation, providing theoretical guidance to accomplish sustainability of continuous maize mulching.

Keywords: Arbuscular mycorrhizal fungi; Fungal functional guilds; Plant pathogen; Plastic film mulch; Soil fungi.