In this study, a heterotrophic/biofilm-electrode autotrophic denitrification reactor (HAD-BER) was constructed and nano-ɑ-Fe2 O3 was coated on granular activated carbon (GAC) as a third electrode to enhance the nitrate removal performance. The introduction of nano-ɑ-Fe2 O3 could stimulate microorganisms to secrete more extracellular polymeric substances (EPS), accelerating the electron transfer. Moreover, more denitrification bacteria were enriched on the particle electrodes, especially Pseudomonas and Thermomonas, which played a significant role in denitrification. The denitrification performance at different COD/N ratios (0.65-3.23) and current intensities (0-150 mA) was investigated in depth. When the nitrate concentration of the influent was 60 mg/L, nitrate was almost completely removed at the optimal current intensity (60 mA) and COD/N ratio (1.29). At the same time, there was almost no nitrite (<0.10 mg/L) and ammonia nitrogen (0 mg/L) accumulation in the effluent. This study provided a new direction for the advancement of HAD-BER and accelerated its implementation. PRACTITIONER POINTS: By introducing nano-a-Fe2O3 into HAD-BER, more denitrification bacteria were enriched on the particle electrodes. The increased contents of polysaccharide and protein content could accelerate the electron transfer. Almost completely denitrification could be achieved at current = 60 mA and COD/N = 1.29. The study provided a new direction for the further development of HAD-BERs.
Keywords: COD/N ratio; HAD-BER; Nano-ɑ-Fe2O3; current intensity; nitrate removal.
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