Lipidomic analysis and triglyceride profiles of fish oil: Preparation through silica gel column and enzymatic treatment

Rongzhen Song; Xichang Wang; Shanggui Deng; Ningping Tao

doi:10.1016/j.foodres.2022.112100

Lipidomic analysis and triglyceride profiles of fish oil: Preparation through silica gel column and enzymatic treatment

Food Res Int. 2022 Dec;162(Pt B):112100. doi: 10.1016/j.foodres.2022.112100. Epub 2022 Nov 2.

Authors

Rongzhen Song¹, Xichang Wang², Shanggui Deng³, Ningping Tao⁴

Affiliations

¹ College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, PR China.
² College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, PR China; Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation, Shanghai, PR China.
³ College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, Zhejiang, PR China.
⁴ College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, PR China; Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation, Shanghai, PR China. Electronic address: nptao@shou.edu.cn.

PMID: 36461340
DOI: 10.1016/j.foodres.2022.112100

Abstract

A comprehensive study of lipidomic coupled with triglyceride profiles onto four fish oils was performed through ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS). Overall, 1010 lipids belonging to 6 categories and 38 lipid classes were identified. Triglycerides (TGs) were the dominant component in four fish oils (40 %-99 % of total lipids), and glycerophospholipids (GPs) and sphingolipids (SLs) were another two major lipid categories in the fish oil (TG50) which prepared through silica gel column. These results revealed that enzymatic treatment has slight effect on lipid distribution but silica gel column could change the lipids composition. TGs composition of four fish oils were separated completely, and the most TG molecule in TG50 is TG(18:3_14:0_18:0), possessed 13.03 ± 5.07 % relative content, these results implied that silica gel column could protect the nature structure of TGs from destroying which may also limited to further improve eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA) purity, but enzymic method was not restricted by this.

Keywords: Enzymic method; Lipidomic; Silica gel column; Triglyceride; UPLC-ESI-MS/MS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fish Oils*
Lipidomics*
Silica Gel
Tandem Mass Spectrometry
Triglycerides

Substances

Fish Oils
Triglycerides
Silica Gel