Revealing color change and drying mechanisms of pulsed vacuum steamed Cistanche deserticola through bioactive components, microstructural and starch gelatinization properties

Abstract

Cistanche deserticola is a famous herbal medicine and has been used worldwide for its kidney-tonifying and anti-aging values. This study investigated the effects of pulsed vacuum steaming (PVS) on bioactive phenylethanoid glycosides (PhGs), total soluble sugars, polysaccharides, color, drying characteristics, microstructure, and starch gelatinization properties of Cistanche deserticola. PVS pretreatment significantly increased PhGs and soluble sugar content while reduced the polysaccharides content. And increasing the material core temperature to 75 °C at the largest diameter was proposed as the optimal steaming condition and the PhGs content was increased by 1.11 times compared with that by atmospheric steaming. The color of steamed samples changed to oily black due to Maillard reaction. PhGs content was significantly (P < 0.05) positively correlated with total color difference (ΔE). Steaming until the ΔE value of 15.95 could achieve the maximum accumulation of PhGs, corresponding to the highest increasing ratio of echinacoside and acteoside. Starch was completely gelatinized and formed a barrier layer adhering to the cell surface when the material core temperature reached 75 °C at the largest diameter, explaining why after steaming the Cistanche deserticola drying time was prolonged by 85.71 %. The study can provide an innovative steaming technology and optimal process parameters for obtaining high-quality Cistanche deserticola decoction pieces, as well as propose a non-destructive testing method to quickly predict PhGs content based on color parameters during the steaming process.

Keywords: Cistanche deserticola; Drying time; Maillard reaction; Microstructure; Physicochemical quality; Pulsed vacuum steaming; Starch gelatinization.