Dummy molecularly imprinted solid-phase extraction-SERS determination of AFB1 in peanut

Li Fan; Qiong Zhang; Feng Wang; Haifeng Yang

doi:10.1016/j.saa.2022.122130

Dummy molecularly imprinted solid-phase extraction-SERS determination of AFB1 in peanut

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Mar 5:288:122130. doi: 10.1016/j.saa.2022.122130. Epub 2022 Nov 28.

Authors

Li Fan¹, Qiong Zhang¹, Feng Wang², Haifeng Yang³

Affiliations

¹ The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, and Department of Chemistry, Shanghai Normal University, Shanghai 200234, PR China.
² The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, and Department of Chemistry, Shanghai Normal University, Shanghai 200234, PR China. Electronic address: wangfeng@shnu.edu.cn.
³ The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, and Department of Chemistry, Shanghai Normal University, Shanghai 200234, PR China. Electronic address: hfyang@shnu.edu.cn.

PMID: 36459720
DOI: 10.1016/j.saa.2022.122130

Abstract

As a class I carcinogen, aflatoxin B1 (AFB1) contamination in foods and feeds accounts for 75 % of the total mycotoxin contamination. In this work, a simple and reliable surface-enhanced Raman spectroscopy (SERS) method for sensitive and selective detection of AFB1 in peanut samples integrated with dummy molecularly imprinted polymers (DMIPs) is developed. N-isopropylacrylamide (NIPAM) and 7-ethoxycoumarin (7-EOC) are chosen as monomer and dummy template, respectively and their ratio was screened through molecular design in both of kinetic and static adsorption views to form the optimal DMIPs. As-prepared dummy molecularly imprinted solid-phase extraction (DMISPE) could selectively enrich AFB1 from peanut samples. Finally, a liquid-liquid interface self-assembly constructed thioctic acid-decorated AgNPs monolayer film (TA-AgNPs MF) as a SERS-active substrate is employed to determine the amount of AFB1 eluted from DMISPE. SERS assay shows high detection sensitivity for AFB1 in peanut samples with limit of detection of 0.1 μg L^-1 and a linear concentration relationship range from 0.1 to 10 μg L^-1.

Keywords: AFB1; DMISPE; Discovery Studio; SERS.

MeSH terms

Adsorption
Aflatoxin B1* / analysis
Arachis
Chromatography, High Pressure Liquid / methods
Molecular Imprinting* / methods
Polymers / chemistry
Solid Phase Extraction / methods

Substances

Aflatoxin B1
Polymers