MiR-146a and miR-155 polymorphisms in Egyptian patients with Behcet's disease

Olfat G Shaker; Omayma O Abdelaleem; Nermeen A Fouad; Naglaa A Ahmed; Hoda A Hussein; Enas G Ibrahem; Abdelrahmaan A Mohamed; Othman M Ahmed; Doaa Y Ali

doi:10.5114/aoms/105349

MiR-146a and miR-155 polymorphisms in Egyptian patients with Behcet's disease

Arch Med Sci. 2021 Mar 19;18(6):1467-1474. doi: 10.5114/aoms/105349. eCollection 2022.

Authors

Olfat G Shaker¹, Omayma O Abdelaleem², Nermeen A Fouad³, Naglaa A Ahmed⁴, Hoda A Hussein⁵, Enas G Ibrahem⁶, Abdelrahmaan A Mohamed⁶, Othman M Ahmed⁷, Doaa Y Ali⁷

Affiliations

¹ Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
² Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Faiyum, Egypt.
³ Department of Rheumatology and Rehabilitation, Faculty of Medicine, Fayoum University, Faiyum, Egypt.
⁴ Department of Medical Physiology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
⁵ Department of Internal Medicine, Faculty of Medicine, Fayoum University, Faiyum, Egypt.
⁶ Department of Medical Microbiology and Immunology, Faculty of Medicine, Fayoum University, Faiyum, Egypt.
⁷ Department of Clinical Pathology, Faculty of Medicine, Fayoum University, Faiyum, Egypt.

Abstract

Introduction: The current study was designed to analyze whether polymorphisms of miR-146a and miR-155 are related to Behçet's disease (BD) in the Egyptian population.

Material and methods: A total of 96 unrelated BD patients and 100 healthy subjects were genotyped for miR-146a (rs2910164) and miR-155 (rs767649) using real-time polymerase chain reaction.

Results: The results showed significant elevation in the frequency of rs2910164 GG and CC genotypes in BD patients compared with controls (adjusted OR = 22.156, 95% CI: 4.728-103.818; p < 0.001 and adjusted OR = 40.358, 95% CI: 8.928-182.440; p < 0.001, respectively). Also, the rs2910164 G allele conferred a higher risk of developing BD (adjusted OR = 3.665, 95% CI: 2.013-6.671; p < 0.001). MiR-146a (rs2910164) polymorphism was a risk factor for susceptibility to BD in dominant, recessive and additive models of inheritance (all p < 0.001), while the miR-155 (rs767649) polymorphism was a risk factor in the recessive model only (p = 0.021). GG and CG genotypes of rs2910164 were associated with higher Behcet's disease current activity index (BDCAI) and ocular involvement compared with CC genotype (p = 0.005 and p = 0.004, respectively). Genotype AT of rs767649 was related to higher BDCAI (p = 0.026) compared with TT and AA genotypes.

Conclusions: miR-146a (rs2910164) and miR-155 (rs767649) are likely to play an important role in the Egyptian population in development of BD and also influence disease severity.

Keywords: Behçet’s disease; miR-146a; miR-155; polymorphism.